Difference between revisions of "Part:BBa K902057"
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HpaC is an oxidoreductase capable of recycling FMN to FMNH<sub>2</sub> while converting NADH to NAD. This part is used in conjuctions with the 4S desulfurization pathway to replace DszD. Doing so improves the rate of desulfurization 7-10x.</p><br>
 
HpaC is an oxidoreductase capable of recycling FMN to FMNH<sub>2</sub> while converting NADH to NAD. This part is used in conjuctions with the 4S desulfurization pathway to replace DszD. Doing so improves the rate of desulfurization 7-10x.</p><br>
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</html>[[Image:File:https://static.igem.org/mediawiki/2012/thumb/1/1c/Ucalgary_team_sulfur_4s_enzyme_pathway_diagram.png/700px-Ucalgary_team_sulfur_4s_enzyme_pathway_diagram.png|centre]]
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</html>[[Image:File.https://static.igem.org/mediawiki/2012/thumb/1/1c/Ucalgary_team_sulfur_4s_enzyme_pathway_diagram.png/700px-Ucalgary_team_sulfur_4s_enzyme_pathway_diagram.png|centre]]
  
 
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Revision as of 03:07, 3 October 2012

HpaC- 4-hydroxyphenylacetate 3-monooxygenase reductase subunit

HpaC is an oxidoreductase capable of recycling FMN to FMNH2 while converting NADH to NAD. This part is used in conjuctions with the 4S desulfurization pathway to replace DszD. Doing so improves the rate of desulfurization 7-10x.


File:File.https://static.igem.org/mediawiki/2012/thumb/1/1c/Ucalgary team sulfur 4s enzyme pathway diagram.png/700px-Ucalgary team sulfur 4s enzyme pathway diagram.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 283