Difference between revisions of "Part:BBa K902052"

Revision as of 02:41, 3 October 2012

HBPS desulfinase (DszB) from R. erythropolis (mutated for improved activity) This sequence codes for HBPS desulfinase (DszB), which is a monomeric enzyme that carries out the last step of the 4S desulfurization pathway. This step carries out the conversion of 2-hydroxybiphenyl-2-sulfinic acid (HBPS) to 2-hydroxybiphenyl, releasing the sulfur in the form of sulfite. Naturally, this enzyme has the lowest activity of all the desulfurization genes involved in the 4S pathway, and thus this step is the rate limiting step. Because of this, the sequence has been mutated Y63F to increase enzyme activity.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 426
Illegal XhoI site found at 418
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 133
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K902052 short</partinfo>
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+This sequence codes for HBPS desulfinase (DszB), which is a monomeric enzyme that carries out the last step of the 4S desulfurization pathway. This step carries out the conversion of 2-hydroxybiphenyl-2-sulfinic acid (HBPS) to 2-hydroxybiphenyl, releasing the sulfur in the form of sulfite. Naturally, this enzyme has the lowest activity of all the desulfurization genes involved in the 4S pathway, and thus this step is the rate limiting step. Because of this, the sequence has been mutated Y63F to increase enzyme activity.<br></html>
+[[Image:Ucalgary2012 DszBpathway figure.PNG|centre]]
-DszB - HBPS desulfinase which carries out the last step of the 4S pathway, converting 2-hydroxybiphenyl-2-sulfinic acid (HBPS) to 2-hydroxybiphenyl. The sequence has been mutated Y63F to increase enzyme activity.
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