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Terminator of the MFA2 gene from Saccharomyces Cerevisiae. It confers relative instability to the mRNA produced, increasing the amount of mRNA made and protein produced.
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Terminator of the MFA2 gene from Saccharomyces Cerevisiae. It confers relative instability to the mRNA produced, increasing the amount of mRNA made and protein produced. [[https://www.jstage.jst.go.jp/article/bbb/75/11/75_110246/_pdf]] "TPS1 Terminator Increases mRNA and Protein Yield in a Saccharomyces Cerevisiae Expression System"
  
 
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Revision as of 02:16, 3 October 2012

MFA2 Terminator

Terminator of the MFA2 gene from Saccharomyces Cerevisiae. It confers relative instability to the mRNA produced, increasing the amount of mRNA made and protein produced. [[1]] "TPS1 Terminator Increases mRNA and Protein Yield in a Saccharomyces Cerevisiae Expression System"

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 217

Plasmid Map

MFA2t plasmid.png

Sequencing

MFA2t sequence.png

This is the sequencing result for the MFA2 gene terminator. As can be seen, it is a perfect match to the genomic DNA terminator.