Difference between revisions of "Part:BBa K804013"
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<partinfo>BBa_K804013 short</partinfo> | <partinfo>BBa_K804013 short</partinfo> | ||
− | This part contains a rhamnose inducible (pRha) | + | This part contains a rhamnose inducible (pRha) MetA coding gene. Upon induction with rhamnose, MetA is expressed for for the biosynthesis of de novo methionine. It encodes for the MetA enzyme which transfers a succinyl group from succinyl-CoA to homoserine, forming O-succinyl-L-homoserine, a precursor of methionine. This construct can be used to induce growth in Met- auxotrophs. |
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Latest revision as of 01:03, 3 October 2012
Rhamnose Inducible MetA coding gene
This part contains a rhamnose inducible (pRha) MetA coding gene. Upon induction with rhamnose, MetA is expressed for for the biosynthesis of de novo methionine. It encodes for the MetA enzyme which transfers a succinyl group from succinyl-CoA to homoserine, forming O-succinyl-L-homoserine, a precursor of methionine. This construct can be used to induce growth in Met- auxotrophs.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]