Difference between revisions of "Part:BBa K741005:Design"

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(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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It is a fusion protein of Cro and GFP. The linker is GGSG (BBa_K648005).
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We construct this part by overlap PCR. The primer we design can combine cro and gfp with a linker [GGSG (BBa_K648005)] and add an RBS and a terminator to it.
  
 
===Source===
 
===Source===

Revision as of 00:58, 3 October 2012

Fusion Protein: crogfp


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 63
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 857


Design Notes

We construct this part by overlap PCR. The primer we design can combine cro and gfp with a linker [GGSG (BBa_K648005)] and add an RBS and a terminator to it.

Source

BBa_K648005 BBa_I13504

References