Difference between revisions of "Part:BBa K741005"
 
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It is a fusion protein of Cro and GFP. The linker between the two protein is GGSG (BBa_K648005).
 
It is a fusion protein of Cro and GFP. The linker between the two protein is GGSG (BBa_K648005).
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We use this part to test the ability of anticro to repress the expression of cro, for crogfp can both be repressed by asRNA of cro and emit green fluorescence.
  
 
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Latest revision as of 00:44, 3 October 2012

Fusion Protein: crogfp

It is a fusion protein of Cro and GFP. The linker between the two protein is GGSG (BBa_K648005). We use this part to test the ability of anticro to repress the expression of cro, for crogfp can both be repressed by asRNA of cro and emit green fluorescence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 63
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 857