Difference between revisions of "Part:BBa K783055"

Revision as of 00:17, 3 October 2012

Level 0 MoClo Destination Vector with AB

This is a Level 0 MoClo destination vector with flanking sites A on the 5' side and site B on the 3' side of the alpha fragment of lacZ.

The fusion site letters refer to 4bp fusion sites: A = GGAG; E = GCTT; B = TACT; F = CGCT; C = AATG; G = TGCC; D = AGGT; H = ACTA.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 251
Illegal XbaI site found at 278
Illegal SpeI site found at 1
Illegal SpeI site found at 7
Illegal SpeI site found at 535
Illegal PstI site found at 290
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 251
Illegal SpeI site found at 1
Illegal SpeI site found at 7
Illegal SpeI site found at 535
Illegal PstI site found at 290
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 251
Illegal BamHI site found at 272
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 251
Illegal XbaI site found at 278
Illegal SpeI site found at 1
Illegal SpeI site found at 7
Illegal SpeI site found at 535
Illegal PstI site found at 290
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 251
Illegal XbaI site found at 278
Illegal SpeI site found at 1
Illegal SpeI site found at 7
Illegal SpeI site found at 535
Illegal PstI site found at 290
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 14
Illegal BsaI.rc site found at 528

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K783055 short</partinfo>
@@ Line 6: / Line 5: @@
 The fusion site letters refer to 4bp fusion sites:
-A	GGAG	E	GCTT
+A	= GGAG;	E	= GCTT;
-B	TACT	F	CGCT
+B	= TACT;	F	= CGCT;
-C	AATG	G	TGCC
+C	= AATG;	G	= TGCC;
-D	AGGT	H	ACTA
+D	= AGGT;	H	= ACTA.