Difference between revisions of "Part:BBa K741001:Design"

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We think it improved BBa_P0451(RBS-cI-Terminator) in parts.igem.
 
We think it improved BBa_P0451(RBS-cI-Terminator) in parts.igem.
  
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However, we think the lac promoter cannot work well on pSB1C3 for there is not lacI gene on it. And K12 strains can not express enough lacI to repress the expression of plac. So the leak expression of lac promoter is quite high. We use glucose in our later experiments to repress plac. But the results are not that good. See our results in BBa K741002 [https://parts.igem.org/Part:BBa_K741002].
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However, we think the lac promoter cannot work well on pSB1C3 for there is not lacI gene on it. And K12 strains can not express enough lacI to repress the expression of plac. So the leak expression of lac promoter is quite high. We use glucose in our later experiments to repress plac. But the results are not that good. See our results in BBa_K741002 [https://parts.igem.org/Part:BBa_K741002].
  
 
===Source===
 
===Source===

Latest revision as of 23:34, 2 October 2012

plac-RBS-cI-T


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We think it improved BBa_P0451(RBS-cI-Terminator) in parts.igem.

However, we think the lac promoter cannot work well on pSB1C3 for there is not lacI gene on it. And K12 strains can not express enough lacI to repress the expression of plac. So the leak expression of lac promoter is quite high. We use glucose in our later experiments to repress plac. But the results are not that good. See our results in BBa_K741002 [1].

Source

BBa_R0010 BBa_P0451

References