Difference between revisions of "Part:BBa K844000"
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10x-Histidine tag with double stop codon TAATAA to allow for better extraction of tagged products and protein termination in a single part. | 10x-Histidine tag with double stop codon TAATAA to allow for better extraction of tagged products and protein termination in a single part. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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| + | This part is a 10x-Histidine tag with two stop codons on the 3’ end. It is intended to be cloned downstream of a protein or protein subunit lacking a stop codon; the 10x-Histidine tag can then be used to extract the protein using Nickel-based affinity columns. Proteins on gels or in cells can also be analyzed with His-tag specific antibodies or dyes. | ||
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| + | The 10x His tag conveys increased binding affinity for nickel resin compared to traditional 6x His tags as shown in [citation needed]. | ||
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| + | IMPORTANT NOTE: Any dyes, antibodies, affinity resins, etc. designed for use with 6x-Histidine tags '''will''' work with this 10x-Histidine tag, and in most cases should work better. | ||
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| + | ===References=== | ||
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| + | [citation needed] | ||
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Revision as of 17:28, 2 October 2012
10x-Histidine (10x-His) Tag with double stop codon (TAATAA)
10x-Histidine tag with double stop codon TAATAA to allow for better extraction of tagged products and protein termination in a single part.
Usage and Biology
This part is a 10x-Histidine tag with two stop codons on the 3’ end. It is intended to be cloned downstream of a protein or protein subunit lacking a stop codon; the 10x-Histidine tag can then be used to extract the protein using Nickel-based affinity columns. Proteins on gels or in cells can also be analyzed with His-tag specific antibodies or dyes.
The 10x His tag conveys increased binding affinity for nickel resin compared to traditional 6x His tags as shown in [citation needed].
IMPORTANT NOTE: Any dyes, antibodies, affinity resins, etc. designed for use with 6x-Histidine tags will work with this 10x-Histidine tag, and in most cases should work better.
References
[citation needed]
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]