Difference between revisions of "Part:BBa K771405"

Latest revision as of 16:26, 2 October 2012

split EGFP 2 with Membrane Anchor 5(without VVD)

Membrane anchor 5 without VVD, which consists of GBD ligand(BBa_K771106) and membrane protein Lgt(BBa_K771102). Membrane Anchor 5 (with and without VVD) is proved to constitutively dimerize with Membrane Anchor [BBa_K771004].

We proved the constitutive interaction between membrane anchor 4 and 5 by fusing split EGFP to membrane anchor 4 and membrane anchor 5 without VVD.

Membrane Anchor 4 and Membrane Anchor 5 without VVD

We fused split EGFP 1 and 2 to Membrane Anchor 4 and Membrane Anchor 5 without VVD to test whether Membrane Anchor 4 and Membrane Anchor 5 without VVD could constitutively aggregate. Proteins in control group are not expected to aggregate like in experimental group.We coexpressed proteins in experimental group and control group respectively in E.coli. After induction for 6 hours, bacteria samples were taken for fluorescence observationThe result shows that Membrane Anchor 4 and Membrane Anchor 5 without VVD could constitutively aggregate through GBD domain and ligand.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 884
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 505

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K771405 short</partinfo>
-M4-EGFP2
+Membrane anchor 5 without VVD, which consists of GBD ligand([https://parts.igem.org/wiki/index.php?title=Part:BBa_K771106 BBa_K771106]) and membrane protein Lgt([https://parts.igem.org/wiki/index.php?title=Part:BBa_K771102 BBa_K771102]). Membrane Anchor 5 (with and without VVD) is proved to constitutively dimerize with Membrane Anchor [[https://parts.igem.org/wiki/index.php?title=Part:BBa_K771004 BBa_K771004]].
+We proved the constitutive interaction between membrane anchor 4 and 5 by fusing split EGFP to membrane anchor 4 and membrane anchor 5 without VVD.
+===Membrane Anchor 4 and Membrane Anchor 5 without VVD===
+[[Image:12SJTU_splitegfp45.png|400px|center]]
+[[Image:12SJTU Standard GFP 2.jpg|400px|center]]
+We fused split EGFP 1 and 2 to Membrane Anchor 4 and Membrane Anchor 5 without VVD to test whether Membrane Anchor 4 and Membrane Anchor 5 without VVD could constitutively aggregate. Proteins in control group are not expected to aggregate like in experimental group.We coexpressed proteins in experimental group and control group respectively in ''E.coli''. After induction for 6 hours, bacteria samples were taken for fluorescence observationThe result shows that Membrane Anchor 4 and Membrane Anchor 5 without VVD could constitutively aggregate through GBD domain and ligand.
 <!-- Add more about the biology of this part here