Difference between revisions of "Part:BBa K779120:Design"
(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
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Modifications: 5' RQ quencher, 3' Alexa fluorophore, all bases modified with 2'O Methyl (ordered as a DNA oligo with Us instead of Ts).<br/>
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'''Modifications:''' 5' Iowa Black RQ quencher, 3' Alexa 488 fluorophore, all bases modified with 2'O Methyl (standard modification for increased stability of RNA); ordered as a DNA oligo with Us instead of Ts.
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IDT DNA Oligo Order: 5IAbRQ/mCmAmU mAmAmCmAmCmAmAmUmC mAmCmA/3AlexF488N/
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'''IDT DNA Oligo Order:''' 5IAbRQ/mCmAmUmAmAmCmAmCmAmAmUmCmAmCmA/3AlexF488N/
  
 
===Source===
 
===Source===

Latest revision as of 06:11, 2 October 2012

RNA Reporter top strand with quencher (RQ) and tag fluorophore (Alexa 488) MammoBlock


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Modifications: 5' Iowa Black RQ quencher, 3' Alexa 488 fluorophore, all bases modified with 2'O Methyl (standard modification for increased stability of RNA); ordered as a DNA oligo with Us instead of Ts.

IDT DNA Oligo Order: 5IAbRQ/mCmAmUmAmAmCmAmCmAmAmUmCmAmCmA/3AlexF488N/

Source

Qian L., Winfree E. Scaling up digital circuit computation with DNA displacement cascades., Science. 2011 Jun 3; 332 (6034):1196-201

References