Difference between revisions of "Part:BBa K779117:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | '''Modifications:''' 5' Iowa Black RQ quencher, all bases modified with 2'O Methyl (ordered as a DNA oligo with Us instead of Ts | + | '''Modifications:''' 5' Iowa Black RQ quencher, all bases modified with 2'O Methyl (standard modification for increased stability of RNA); ordered as a DNA oligo with Us instead of Ts. |
'''IDT DNA Oligo Order:''' /5IAbRQ/ mCmAmUmAmAmCmAmCmAmAmUmCmAmCmA | '''IDT DNA Oligo Order:''' /5IAbRQ/ mCmAmUmAmAmCmAmCmAmAmUmCmAmCmA |
Latest revision as of 06:10, 2 October 2012
RNA Reporter top strand with quencher (RQ) MammoBlock
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Modifications: 5' Iowa Black RQ quencher, all bases modified with 2'O Methyl (standard modification for increased stability of RNA); ordered as a DNA oligo with Us instead of Ts.
IDT DNA Oligo Order: /5IAbRQ/ mCmAmUmAmAmCmAmCmAmAmUmCmAmCmA
Source
Qian L., Winfree E. Scaling up digital circuit computation with DNA displacement cascades., Science. 2011 Jun 3; 332 (6034):1196-201