Difference between revisions of "Part:BBa K779503:Design"

 
(→‎Design Notes)
 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K779503 short</partinfo>
 
<partinfo>BBa_K779503 short</partinfo>
Line 7: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
3' phosphate added to protect from some 3' exonucleases (suggested by IDT).
+
3' phosphate added to protect from some 3' exonucleases (suggested by IDT). 5' IR fluorophore added for quantifying oligo levels.
−
 
+
−
See more details at the MIT iGEM wiki: http://2012.igem.org/Team:MIT
+
  
 +
Sequence designed to be orthogonal to the HEK293 transcriptome, and to have a high melting temperature. The latter was achieved by using a high G/C content (constrained to be less than 70% by [http://www.sciencemag.org/content/332/6034/1196.abstract Qian ''et al.'' 2011]).
  
 +
See more details on design principles with respect to orthogonality at the [http://2012.igem.org/Team:MIT/ResultsSensing#sensing1bio MIT iGEM wiki].
  
 
===Source===
 
===Source===

Latest revision as of 05:28, 2 October 2012

Long input strand MammoBlock


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

3' phosphate added to protect from some 3' exonucleases (suggested by IDT). 5' IR fluorophore added for quantifying oligo levels.

Sequence designed to be orthogonal to the HEK293 transcriptome, and to have a high melting temperature. The latter was achieved by using a high G/C content (constrained to be less than 70% by [http://www.sciencemag.org/content/332/6034/1196.abstract Qian et al. 2011]).

See more details on design principles with respect to orthogonality at the [http://2012.igem.org/Team:MIT/ResultsSensing#sensing1bio MIT iGEM wiki].

Source

De novo design.

References