Difference between revisions of "Part:BBa K779502:Design"

 
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===Design Notes===
 
===Design Notes===
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Sequence designed to be orthogonal to the HEK293 transcriptome, and to have a high melting temperature.
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Sequence designed to be orthogonal to the HEK293 transcriptome, and to have a high melting temperature. The latter was achieved by using a high G/C content (constrained to be less than 70% by [http://www.sciencemag.org/content/332/6034/1196.abstract Qian ''et al.'' 2011]).
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See more details at the MIT iGEM wiki: http://2012.igem.org/Team:MIT
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See more details on design principles with respect to orthogonality at the [http://2012.igem.org/Team:MIT/ResultsSensing#sensing1bio MIT iGEM wiki].
  
 
===Source===
 
===Source===

Latest revision as of 05:26, 2 October 2012

Long reporter bottom strand MammoBlock


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequence designed to be orthogonal to the HEK293 transcriptome, and to have a high melting temperature. The latter was achieved by using a high G/C content (constrained to be less than 70% by [http://www.sciencemag.org/content/332/6034/1196.abstract Qian et al. 2011]).

See more details on design principles with respect to orthogonality at the [http://2012.igem.org/Team:MIT/ResultsSensing#sensing1bio MIT iGEM wiki].

Source

De novo design.

References