Difference between revisions of "Part:BBa K779502"
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Long reporter bottom strand to bind to [[Part:BBa_K779501]] and [[Part:BBa_K779500]].
 
Long reporter bottom strand to bind to [[Part:BBa_K779501]] and [[Part:BBa_K779500]].
  
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Modifications: 3' ROX florophore, all bases modified with 2'O Methyl (ordered as a DNA oligo with Us instead of Ts).
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'''Modifications:''' 3' ROX florophore, all bases modified with 2'O Methyl (ordered as a DNA oligo with Us instead of Ts).
  
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IDT DNA oligo order: mUmGmG mAmUmA mGmUmU mGmGmU mGmGmG mAmGmA mGmGmA mGmUmG mGmGmU mG/3ROX_N/
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'''IDT DNA Oligo Order:''' mUmGmGmAmUmAmGmUmUmGmGmUmGmGmGmAmGmAmGmGmAmGmUmGmGmGmUmG/3ROX_N/
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This is an improved version of [[Part:BBa_K779116]], with this design addressing the low melting temperature, orthogonality and small length of the previous iteration.
  
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
  
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<!-- -->
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Provides a quenchable fluorophore. When annealed to a top strand (see parts above), the ROX fluorophore is quenched by an Iowa Black RQ quencher. When an input strand ([[Part:BBa_K779503]]) is added, strand displacement occurs and the quencher top strand is released.
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<span class='h3bb'>Sequence and Features</span>
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===Sequence and Features===
 
<partinfo>BBa_K779502 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K779502 SequenceAndFeatures</partinfo>
  

Revision as of 05:25, 2 October 2012

Long reporter bottom strand MammoBlock

Long reporter bottom strand to bind to Part:BBa_K779501 and Part:BBa_K779500.

Modifications: 3' ROX florophore, all bases modified with 2'O Methyl (ordered as a DNA oligo with Us instead of Ts).

IDT DNA Oligo Order: mUmGmGmAmUmAmGmUmUmGmGmUmGmGmGmAmGmAmGmGmAmGmUmGmGmGmUmG/3ROX_N/

This is an improved version of Part:BBa_K779116, with this design addressing the low melting temperature, orthogonality and small length of the previous iteration.

Usage and Biology

Provides a quenchable fluorophore. When annealed to a top strand (see parts above), the ROX fluorophore is quenched by an Iowa Black RQ quencher. When an input strand (Part:BBa_K779503) is added, strand displacement occurs and the quencher top strand is released.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]