Difference between revisions of "Part:BBa K779500:Design"

(→‎Design Notes)
(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
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4-nucleotide bulge added to [[Part:BBa_K779501]]. Bulge placed 10-nt from the 3' end as a potential protection mechanism from cleavage by RISC as suggested by Iba et al. (2011).
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4-nucleotide bulge added to [[Part:BBa_K779501]]. Bulge placed 10-nt from the 3' end as a potential protection mechanism from cleavage by RISC as suggested by [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3333889/ Iba ''et al.'' 2011]. See [[Part:BBa K779501]] for sequence design besides the bulge.
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See more details at the MIT iGEM wiki: http://2012.igem.org/Team:MIT
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===Source===
 
===Source===

Latest revision as of 05:17, 2 October 2012

Long reporter top strand with bulge MammoBlock


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

4-nucleotide bulge added to Part:BBa_K779501. Bulge placed 10-nt from the 3' end as a potential protection mechanism from cleavage by RISC as suggested by [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3333889/ Iba et al. 2011]. See Part:BBa K779501 for sequence design besides the bulge.

Source

De novo design.

References