Difference between revisions of "Part:BBa K779501:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | Sequence designed to be orthogonal to the HEK293 transcriptome, and to have a high melting temperature. | + | Sequence designed to be orthogonal to the HEK293 transcriptome, and to have a high melting temperature. The latter was achieved by using a high G/C content (constrained to be less than 70% by [http://www.sciencemag.org/content/332/6034/1196.abstract Qian ''et al.'' 2011]). |
See more details on design principles with respect to orthogonality at the [http://2012.igem.org/Team:MIT/ResultsSensing#sensing1bio MIT iGEM wiki]. | See more details on design principles with respect to orthogonality at the [http://2012.igem.org/Team:MIT/ResultsSensing#sensing1bio MIT iGEM wiki]. |
Latest revision as of 05:14, 2 October 2012
Long reporter top strand MammoBlock
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Sequence designed to be orthogonal to the HEK293 transcriptome, and to have a high melting temperature. The latter was achieved by using a high G/C content (constrained to be less than 70% by [http://www.sciencemag.org/content/332/6034/1196.abstract Qian et al. 2011]).
See more details on design principles with respect to orthogonality at the [http://2012.igem.org/Team:MIT/ResultsSensing#sensing1bio MIT iGEM wiki].
Source
De novo design.