Difference between revisions of "Part:BBa K779501"
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<partinfo>BBa_K779501 short</partinfo>
 
<partinfo>BBa_K779501 short</partinfo>
  
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Long reporter top strand to bind to [[Part:BBa K779502]].
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Long reporter top strand to be annealed with [[Part:BBa K779502]].
  
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Modifications: 5' RQ quencher, 3' Alexa florophore, all bases modified with 2'O Methyl (ordered as a DNA oligo with Us instead of Ts).
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'''Modifications:''' 5' Iowa Black RQ quencher, 3' Alexa 488 florophore, all bases modified with 2'O Methyl (ordered as a DNA oligo with Us instead of Ts).
  
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IDT DNA oligo order: /5IAbRQ/mCmA mCmCmC mAmCmU mCmCmU mCmUmC mCmCmA mCmCmA /3AlexF488N/
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'''IDT DNA oligo order:''' /5IAbRQ/mCmAmCmCmCmAmCmUmCmCmUmCmUmCmCmCmAmCmCmA/3AlexF488N/
  
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
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When annealed to [[Part:BBa K779502]] acts as a reporter for strand displacement reactions.
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<span class='h3bb'>Sequence and Features</span>
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===Sequence and Features===
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Sequence is designed to be orthogonal to the HEK293 transcriptome (see Design). Design is improved on the part [[Part:BBa_K779121]], with goals being a higher melting temperature, more orthogonal and longer domain.
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<partinfo>BBa_K779501 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K779501 SequenceAndFeatures</partinfo>
  

Revision as of 05:11, 2 October 2012

Long reporter top strand MammoBlock

Long reporter top strand to be annealed with Part:BBa K779502.

Modifications: 5' Iowa Black RQ quencher, 3' Alexa 488 florophore, all bases modified with 2'O Methyl (ordered as a DNA oligo with Us instead of Ts).

IDT DNA oligo order: /5IAbRQ/mCmAmCmCmCmAmCmUmCmCmUmCmUmCmCmCmAmCmCmA/3AlexF488N/

Usage and Biology

When annealed to Part:BBa K779502 acts as a reporter for strand displacement reactions.


Sequence and Features

Sequence is designed to be orthogonal to the HEK293 transcriptome (see Design). Design is improved on the part Part:BBa_K779121, with goals being a higher melting temperature, more orthogonal and longer domain.



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]