Difference between revisions of "Part:BBa K779500"

Revision as of 05:07, 2 October 2012

Long reporter top strand with bulge MammoBlock

Long reporter top strand with a 4 nucleotide bulge, binds to Part:BBa K779502.

Modifications: 5' Iowa Black RQ quencher, 3' Alexa 488 florophore, all bases modified with 2'O Methyl (ordered as a DNA oligo with Us instead of Ts).

IDT DNA oligo order: /5IAbRQ/mCmAmCmCmCmAmCmUmCmCmCmAmCmUmUmCmUmCmCmCmAmCmCmA/3AlexF488N/

Usage and Biology

Used as an alternative quencher (top) strand to Part:BBa K779501. The only difference is a 4 nucleotide bulge, which is supposed to protect the annealed complex (below) from degradation by the RISC complex (see the Design section).

When annealed to Part:BBa K779502 forms a tagged reporter for strand displacement reactions (input Part:BBa_K779503, negative control Part:BBa_K779504).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K779500 short</partinfo>
-Long reporter top strand with a 4nt bulge, binds to [[Part:BBa K779502]].
+Long reporter top strand with a 4 nucleotide bulge, binds to [[Part:BBa K779502]].
 '''Modifications:''' 5' Iowa Black RQ quencher, 3' Alexa 488 florophore, all bases modified with 2'O Methyl (ordered as a DNA oligo with Us instead of Ts).