Difference between revisions of "Part:BBa K799005"
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===Plasmid Map=== | ===Plasmid Map=== | ||
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===Sequencing=== | ===Sequencing=== | ||
Revision as of 04:58, 2 October 2012
Ethanol/Stress Inducible YML070W Promoter
YML070W promoter shows an increase in protein expression when exposed to up to 14% ethanol.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Characterization
This is fluorescence data for the expression of GFP under the control of YMR170C, our ethanol induced promoter. Fluorescence can be seen soon after ethanol induction. Furthermore, the fluorescence plateaus but remains significant till our last time point of 230 minutes.
Plasmid Map
Sequencing
This is the sequencing result for the YML070W promoter. As can be seen, it is a perfect match to the genomic DNA promoter.