Difference between revisions of "Part:BBa K779500"

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Long reporter top strand with a 4nt bulge, binds to [[Part:BBa K779502]].
 
Long reporter top strand with a 4nt bulge, binds to [[Part:BBa K779502]].
  
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Modifications: 5' RQ quencher, 3' Alexa florophore, all bases modified with 2'O Methyl (ordered as a DNA oligo with Us instead of Ts).
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'''Modifications:''' 5' Iowa Black RQ quencher, 3' Alexa 488 florophore, all bases modified with 2'O Methyl (ordered as a DNA oligo with Us instead of Ts).
  
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IDT DNA oligo order: /5IAbRQ/mCmA mCmCmC mAmCmU mCmCmC mAmCmU mUmCmU mCmCmC mAmCmC mA/3AlexF488N/
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'''IDT DNA oligo order:''' /5IAbRQ/mCmAmCmCmCmAmCmUmCmCmCmAmCmUmUmCmUmCmCmCmAmCmCmA/3AlexF488N/
  
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
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Used as an alternative quencher (top) strand to [[Part:BBa K779501]]. The only difference is a 4 nucleotide bulge, which is supposed to protect the annealed complex (below) from degradation by the RISC complex (see the Design section).
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When annealed to [[Part:BBa K779502]] forms a tagged reporter for strand displacement reactions (input [[Part:BBa_K779503]], negative control [[Part:BBa_K779504]]).
  
 
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<span class='h3bb'>Sequence and Features</span>
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===Sequence and Features===
 
<partinfo>BBa_K779500 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K779500 SequenceAndFeatures</partinfo>
  

Revision as of 04:42, 2 October 2012

Long reporter top strand with bulge MammoBlock

Long reporter top strand with a 4nt bulge, binds to Part:BBa K779502.

Modifications: 5' Iowa Black RQ quencher, 3' Alexa 488 florophore, all bases modified with 2'O Methyl (ordered as a DNA oligo with Us instead of Ts).

IDT DNA oligo order: /5IAbRQ/mCmAmCmCmCmAmCmUmCmCmCmAmCmUmUmCmUmCmCmCmAmCmCmA/3AlexF488N/

Usage and Biology

Used as an alternative quencher (top) strand to Part:BBa K779501. The only difference is a 4 nucleotide bulge, which is supposed to protect the annealed complex (below) from degradation by the RISC complex (see the Design section).

When annealed to Part:BBa K779502 forms a tagged reporter for strand displacement reactions (input Part:BBa_K779503, negative control Part:BBa_K779504).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]