Difference between revisions of "Part:BBa K779119"
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<partinfo>BBa_K779119 short</partinfo> | <partinfo>BBa_K779119 short</partinfo> | ||
− | Used as a control input to the RNA reporter formed by parts [[Part:BBa_K779116]] and ([[Part:BBa_K779120]] or [[Part:BBa_K779117]]). | + | Used as a control input to the RNA reporter formed by parts [[Part:BBa_K779116]] and ([[Part:BBa_K779120]] or [[Part:BBa_K779117]]). Refer to these part pages for data on [[Part:BBa K779119]]. |
− | + | ||
− | + | '''Modifications:''' all bases modified with 2'O Methyl (ordered as a DNA oligo with Us instead of Ts). | |
+ | |||
+ | '''IDT DNA Oligo Order:''' mCmAmUmCmCmAmUmUmCmCmAmCmUmCmA mUmCmU mCmA | ||
+ | |||
===Usage and Biology=== | ===Usage and Biology=== | ||
+ | Used in concert with the parts mentioned above, it acts as a negative control for a strand displacement reaction (see [[Part:BBa_K77912]] and other related parts). While it has a matching toehold, the recognition domain is non-matching, so strand-displacement will not occur. | ||
<!-- --> | <!-- --> | ||
− | + | ===Sequence and Features=== | |
<partinfo>BBa_K779119 SequenceAndFeatures</partinfo> | <partinfo>BBa_K779119 SequenceAndFeatures</partinfo> | ||
Revision as of 04:30, 2 October 2012
RNA Control input (scrambled) MammoBlock
Used as a control input to the RNA reporter formed by parts Part:BBa_K779116 and (Part:BBa_K779120 or Part:BBa_K779117). Refer to these part pages for data on Part:BBa K779119.
Modifications: all bases modified with 2'O Methyl (ordered as a DNA oligo with Us instead of Ts).
IDT DNA Oligo Order: mCmAmUmCmCmAmUmUmCmCmAmCmUmCmA mUmCmU mCmA
Usage and Biology
Used in concert with the parts mentioned above, it acts as a negative control for a strand displacement reaction (see Part:BBa_K77912 and other related parts). While it has a matching toehold, the recognition domain is non-matching, so strand-displacement will not occur.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]