Difference between revisions of "Part:BBa K799005"

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===Sequencing===
 
===Sequencing===
  
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[[]]
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[[Image:YML070W sequencing.png]]
  
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This is the sequencing result for colony 1. Colony 1 is a perfect match to the genomic DNA promoter.
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This is the sequencing result for the YML070W promoter. As can be seen, it is a perfect match to the genomic DNA promoter.

Revision as of 22:25, 1 October 2012

Ethanol/Stress Inducible YML070W Promoter

YML070W promoter shows an increase in protein expression when exposed to up to 14% ethanol.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterization

ProA3 fluoro.png

This is fluorescence data for the expression of GFP under the control of YMR170C, our ethanol induced promoter. Fluorescence can be seen soon after ethanol induction. Furthermore, the fluorescence plateaus but remains significant till our last time point of 230 minutes.

Plasmid Map

[[]]


Sequencing

YML070W sequencing.png

This is the sequencing result for the YML070W promoter. As can be seen, it is a perfect match to the genomic DNA promoter.