Difference between revisions of "Part:BBa K799001"
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===Characterization===
 
===Characterization===
  
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[[Image:Characterization ProA1.png]]
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[[Image:ProA1 fluoro.png]]
  
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This is fluorescence data for the expression of GFP under the control of YJL200C, our ethanol induced promoter. Fluorescence can be seen soon after ethanol induction. Furthermore, the fluorescence plateaus but remains significant till our last time point of 230 minutes.  
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This is fluorescence data for the expression of GFP under the control of YJL200C, our ethanol induced promoter. Fluorescence can be seen soon after ethanol induction. Furthermore, the fluorescence plateaus but remains significant till our last time point of 230 minutes.
  
 
===Plasmid Map===
 
===Plasmid Map===

Revision as of 21:09, 1 October 2012

Ethanol/Stress Inducible YJL200C Promoter

YJL200C promoter shows an increase in protein expression when exposed up to 14% ethanol.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 172
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 172
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 172
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 172
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterization

ProA1 fluoro.png

This is fluorescence data for the expression of GFP under the control of YJL200C, our ethanol induced promoter. Fluorescence can be seen soon after ethanol induction. Furthermore, the fluorescence plateaus but remains significant till our last time point of 230 minutes.

Plasmid Map

[[]]


Sequencing

[[]]

This is the sequencing result for colony 1. Colony 1 is a perfect match to the genomic DNA promoter.