Difference between revisions of "Part:BBa K917001:Design"
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===Source=== | ===Source=== | ||
| − | This part consists of the E. coli chromosomal ars promoter and arsR repressor gene (J33201), which together comprise a promoter system induced by sodium arsenate, plus the lacZ' minigene (J33202) and the cscA sucrose hydrolase gene from an E. coli O157 strain (K917000). | + | This part consists of the ''E. coli'' chromosomal ars promoter and arsR repressor gene (J33201), which together comprise a promoter system induced by sodium arsenate, plus the lacZ' minigene (J33202) and the ''cscA'' sucrose hydrolase gene from an E. coli O157 strain (K917000). |
===References=== | ===References=== | ||
Jahreis, K., Bentler, L., Bockmann, J., Hans, S., Meyer, A., Siepelmeyer, J., et al. (2002). Adaptation of sucrose metabolism in the Escherichia coli Wild-Type Strain EC31132. Journal of Bacteriology, 5307-5316. | Jahreis, K., Bentler, L., Bockmann, J., Hans, S., Meyer, A., Siepelmeyer, J., et al. (2002). Adaptation of sucrose metabolism in the Escherichia coli Wild-Type Strain EC31132. Journal of Bacteriology, 5307-5316. | ||
Latest revision as of 21:04, 1 October 2012
ars promoter plus cscA sucrose assimilation gene
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1042
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 255
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Made by combining J33203 with K917000
Source
This part consists of the E. coli chromosomal ars promoter and arsR repressor gene (J33201), which together comprise a promoter system induced by sodium arsenate, plus the lacZ' minigene (J33202) and the cscA sucrose hydrolase gene from an E. coli O157 strain (K917000).
References
Jahreis, K., Bentler, L., Bockmann, J., Hans, S., Meyer, A., Siepelmeyer, J., et al. (2002). Adaptation of sucrose metabolism in the Escherichia coli Wild-Type Strain EC31132. Journal of Bacteriology, 5307-5316.