Difference between revisions of "Part:BBa K917002:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | + | The lac promoter and Red Fluorescent Protein (RFP) from BBa_J04450 (but without the terminator) have been added to SacB | |
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===Source=== | ===Source=== |
Revision as of 20:52, 1 October 2012
lac promoter plus RFP and sacB
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 781
Illegal AgeI site found at 893 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The lac promoter and Red Fluorescent Protein (RFP) from BBa_J04450 (but without the terminator) have been added to SacB
Source
From Bacillus subtilis genomic DNA
References
Gay, P., Coq, D. l., Strinmetz, M., Ferrari, E., & Hoch, J. A. (1983). Cloning Structural Gene SacB, which Codes for Exoenzyme Levansucrase of Bacillus subtilis: Expression of the Gene in Esherichia coli. Journal of Bacteriology , 1424-1431. French, C., & Kowal, M. (2010, 09 24). B. subtilis levansucrase. Lethal to E.coli in presence of sucrose. Retrieved 2012, from Registry of standard biological parts: https://parts.igem.org/Part:BBa_K322921