Difference between revisions of "Part:BBa K917000"

Latest revision as of 20:35, 1 October 2012

cscA sucrose hydrolase from E. coli 157:H7 Sakai

The is the gene cscA (Ecs3243) from Escherichia coli O157:H7 strain Sakai, encoding a sucrose hydrolase gene (Jahreis, et al., 2002). K12-derived laboratory strains of E. coli generally can not use sucrose as a carbon source, but it has been reported that expression of this gene alone from the related strain E. coli W is sufficient to allow good growth with sucrose as sole carbon source. This BioBrick includes the native ribosome binding site (overlapping the scar sequence). This biobrick can be used as a selectable marker.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 265
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K917000 short</partinfo>
-The is the gene cscA (Ecs3243) from Escherichia coli O157:H7 strain Sakai, encoding a sucrose hydrolase gene. K12-derived laboratory strains of E. coli generally can not use sucrose as a carbon source, but it has been reported that expression of this gene alone from the related strain E. coli W is sufficient to allow good growth with sucrose as sole carbon source. This BioBrick includes the native ribosome binding site (overlapping the scar sequence).
+The is the gene'' cscA'' (Ecs3243) from ''Escherichia coli'' O157:H7 strain Sakai, encoding a sucrose hydrolase gene (Jahreis, et al., 2002). K12-derived laboratory strains of E. coli generally can not use sucrose as a carbon source, but it has been reported that expression of this gene alone from the related strain ''E. coli'' W is sufficient to allow good growth with sucrose as sole carbon source. This BioBrick includes the native ribosome binding site (overlapping the scar sequence). This biobrick can be used as a selectable marker.
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