Difference between revisions of "Part:BBa J45006"

Revision as of 22:14, 7 June 2006

alcohol acetyltransferase I (ATF1)

From S. cerevisiae (yeast)

The gene ATF1 encodes the enzyme alcohol acetyltransferase I (AATase I), which produces acetate esters from acetyl CoA and alcohols. We will be interested in using AATase I to catalyze the reaction of isoamyl alcohol -> isoamyl acetate; isoamyl acetate is a volatile ester with a strong banana-like scent. (The related enzyme AATase II, which is encoded by the gene ATF2, performs a similar function...but it is less efficient.)

Horton et al. reported a yield of 1.8mM isoamyl acetate 40 hours after the addition of 10mM isoamyl alcohol to cultured E. coli expressing the ATF1 gene.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 99
Illegal BamHI site found at 1426
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 526
Illegal SapI.rc site found at 1534

@@ Line 5: / Line 5: @@
 ===Usage and Biology===
-*The gene ATF1 encodes the enzyme alcohol acetyltransferase 1, which produces acetate esters from acetyl CoA and alcohols.  We will be interested in using ATF1 to catalyze the reaction of isoamyl alcohol -> isoamyl acetate; isoamyl acetate is a volatile ester with a strong banana-like scent.
+*The gene ATF1 encodes the enzyme alcohol acetyltransferase I (AATase I), which produces acetate esters from acetyl CoA and alcohols.  We will be interested in using AATase I to catalyze the reaction of isoamyl alcohol -> isoamyl acetate; isoamyl acetate is a volatile ester with a strong banana-like scent. (The related enzyme AATase II, which is encoded by the gene ATF2, performs a similar function...but it is less efficient.)
 *Horton et al. reported a yield of 1.8mM isoamyl acetate 40 hours after the addition of 10mM isoamyl alcohol to cultured E. coli expressing the ATF1 gene.