Difference between revisions of "Part:BBa K929100"

Latest revision as of 18:03, 1 October 2012

Anti-EGFR scFv425

General information

Anti-EGFR scFv425

BioBrick Nr.	BBa_929100
RFC standard	RFC 25
Requirement	pSB1C3
Source	plasmid pKMEF425bla
Submitted by	[http://2012.igem.org/Team:Potsdam_Bioware Potsdam_Bioware2012]

The single chain fragment (scFV425) specifically binds to the epidermal growth factor receptor (EGFR) domain 3. The part has a shortened N-terminal FLAG-tag sequence of five amino acids (DYKDE) for purification and detection.

Characterization

For characterization and further applications see BBa_K929101.

Usage and Biology

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 538
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 [[Image:UP12_BBa_K929100_vector.png|left|thumb|400px]]<br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br>
 <br>
-The single chain fragment (scFV425) specifically binds to the epidermal growth factor receptor (EGFR) domain 3. The part has a shortened N-terminal FLAG-tag sequence of five amino acids (DYKDE) for purification and detection. For characterisation and further applications see BBa_K9291001.<br>
+The single chain fragment (scFV425) specifically binds to the epidermal growth factor receptor (EGFR) domain 3. The part has a shortened N-terminal FLAG-tag sequence of five amino acids (DYKDE) for purification and detection. <br>
 <br>
-<!-- Add more about the biology of this part here
+<p style="background-color: rgb(238, 221, 130); font-weight: bold;">Characterization</p>
+For characterization and further applications see BBa_K929101.
 ===Usage and Biology===