Difference between revisions of "Part:BBa K929100"
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<partinfo>BBa_K929100 short</partinfo> | <partinfo>BBa_K929100 short</partinfo> | ||
<p style="background-color: rgb(238, 221, 130); font-weight: bold;">General information</p> | <p style="background-color: rgb(238, 221, 130); font-weight: bold;">General information</p> | ||
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{| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right" | {| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right" | ||
| − | ! colspan="2" style="background:rgb(240, 20, 70);"| | + | ! colspan="2" style="background:rgb(240, 20, 70);"|Anti-EGFR scFv425 |
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! colspan="2"|[[Image:UP12_BBa_K929100.png|300px]] | ! colspan="2"|[[Image:UP12_BBa_K929100.png|300px]] | ||
Revision as of 17:31, 1 October 2012
Anti-EGFR scFv425
General information
| Anti-EGFR scFv425 | |
|---|---|
| |
| BioBrick Nr. | BBa_929100 |
| RFC standard | RFC 25 |
| Requirement | pSB1C3 |
| Source | plasmid pKMEF425bla |
| Submitted by | [http://2012.igem.org/Team:Potsdam_Bioware Potsdam_Bioware2012] |
The single chain fragment (scFV425) specifically binds to the epidermal growth factor receptor (EGFR) domain 3. The part has a shortened N-terminal FLAG-tag sequence of five amino acids (DYKDE) for purification and detection.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 538
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]