Difference between revisions of "Part:BBa K945008"
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<partinfo>BBa_K945008 short</partinfo> | <partinfo>BBa_K945008 short</partinfo> | ||
| − | This sequence codes for CrtI (Phytoene desaturase), codon optimized for expression in Pichia pastoris and engineered to include a 6xHis-tag in its N-terminus and the peroxisomal targeting signal type 1 (PTS1) in its C-terminus. | + | This sequence codes for CrtI (Phytoene desaturase), codon optimized for expression in <i>Pichia pastoris</i> and engineered to include a 6xHis-tag in its N-terminus and the peroxisomal targeting signal type 1 (PTS1) in its C-terminus. It also includes a yeast Kozak consensus sequence and the AOX1 transcription terminator sequence for its use in expression. |
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Latest revision as of 08:55, 1 October 2012
Pichia pastoris optimized His-CrtI-PTS1
This sequence codes for CrtI (Phytoene desaturase), codon optimized for expression in Pichia pastoris and engineered to include a 6xHis-tag in its N-terminus and the peroxisomal targeting signal type 1 (PTS1) in its C-terminus. It also includes a yeast Kozak consensus sequence and the AOX1 transcription terminator sequence for its use in expression.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 1892
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 1876
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1861
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 1892
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 1892
Illegal AgeI site found at 1580 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 879
Illegal BsaI.rc site found at 1311
Illegal BsaI.rc site found at 1844