Difference between revisions of "Part:BBa K945006"
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<partinfo>BBa_K945006 short</partinfo> | <partinfo>BBa_K945006 short</partinfo> | ||
| − | This sequence codes for CrtE (GGPP synthase), codon optimized for expression in Pichia pastoris and engineered to include a 6xHis-tag in its N-terminus and the peroxisomal targeting signal type 1 (PTS1) in its C-terminus. | + | This sequence codes for CrtE (GGPP synthase), codon optimized for expression in <i>Pichia pastoris<i/> and engineered to include a 6xHis-tag in its N-terminus and the peroxisomal targeting signal type 1 (PTS1) in its C-terminus. It also includes a yeast Kozak consensus sequence and the AOX1 transcription terminator sequence for its use in expression. |
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Revision as of 08:45, 1 October 2012
Pichia pastoris optimized His-CrtE-PTS1
This sequence codes for CrtE (GGPP synthase), codon optimized for expression in Pichia pastoris<i/> and engineered to include a 6xHis-tag in its N-terminus and the peroxisomal targeting signal type 1 (PTS1) in its C-terminus. It also includes a yeast Kozak consensus sequence and the AOX1 transcription terminator sequence for its use in expression.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 1346
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1318
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 1346
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 1346
Illegal AgeI site found at 1037 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1301