Difference between revisions of "Part:BBa K945007"

 
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<partinfo>BBa_K945007 short</partinfo>
 
<partinfo>BBa_K945007 short</partinfo>
  
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This sequence codes for CrtB (Phytoene synthase), codon optimized for expression in Pichia pastoris and engineered to include a 6xHis-tag in its N-terminus and the peroxisomal targeting signal type 1 (PTS1) in its C-terminus.
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This sequence codes for CrtB (Phytoene synthase), codon optimized for expression in <i>Pichia pastoris</i> and engineered to include a 6xHis-tag in its N-terminus and the peroxisomal targeting signal type 1 (PTS1) in its C-terminus.
  
 
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Revision as of 08:01, 1 October 2012

Pichia pastoris optimized His-CrtB-PTS1

This sequence codes for CrtB (Phytoene synthase), codon optimized for expression in Pichia pastoris and engineered to include a 6xHis-tag in its N-terminus and the peroxisomal targeting signal type 1 (PTS1) in its C-terminus.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1529
    Illegal XbaI site found at 1558
    Illegal PstI site found at 1342
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1529
    Illegal PstI site found at 1342
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1529
    Illegal BamHI site found at 1333
    Illegal BamHI site found at 1364
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1529
    Illegal XbaI site found at 1558
    Illegal PstI site found at 1342
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1529
    Illegal XbaI site found at 1558
    Illegal PstI site found at 1342
    Illegal AgeI site found at 1052
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 976
    Illegal BsaI.rc site found at 1316
    Illegal SapI site found at 14