Difference between revisions of "Part:BBa J45006"
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<partinfo>BBa_J45006 short</partinfo> | <partinfo>BBa_J45006 short</partinfo> | ||
− | + | From S. cerevisiae | |
===Usage and Biology=== | ===Usage and Biology=== | ||
− | + | The gene ATF1 encodes the enzyme alcohol acetyltransferase 1, which produces acetate esters from acetyl CoA and alcohols. We will be interested in using ATF1 to catalyze the reaction of isoamyl alcohol -> isoamyl acetate; the product is of interest because it is a volatile ester with a strong banana-like scent. Horton et al. reported a yield of 1.8mM isoamyl acetate 40 hours after the addition of 10mM isoamyl alcohol to cultured E. coli expressing the ATF1 gene. | |
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> |
Revision as of 21:37, 7 June 2006
alcohol acetyltransferase I (ATF1)
From S. cerevisiae
Usage and Biology
The gene ATF1 encodes the enzyme alcohol acetyltransferase 1, which produces acetate esters from acetyl CoA and alcohols. We will be interested in using ATF1 to catalyze the reaction of isoamyl alcohol -> isoamyl acetate; the product is of interest because it is a volatile ester with a strong banana-like scent. Horton et al. reported a yield of 1.8mM isoamyl acetate 40 hours after the addition of 10mM isoamyl alcohol to cultured E. coli expressing the ATF1 gene.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 99
Illegal BamHI site found at 1426 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 526
Illegal SapI.rc site found at 1534