Difference between revisions of "Part:BBa K861201"
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<partinfo>BBa_K861201 short</partinfo> | <partinfo>BBa_K861201 short</partinfo> | ||
We plan to place endonuclease BglII and YhjH gene downstream the xylR repressed promoter BBa_K861200 to make bacteria die without lysis and lose their adhesion when exposed to xylose. | We plan to place endonuclease BglII and YhjH gene downstream the xylR repressed promoter BBa_K861200 to make bacteria die without lysis and lose their adhesion when exposed to xylose. | ||
| + | Horizontal gene transfer can also be prevented using a two plasmid system. In one plasmid, xlyR repressor encoding gene from Bacillus subtilis will be assembled with constitutively expressed promoter. On the other plasmid, other devices can be coupled with xylR repressed death device. In our GMOs, since death system is suppressed by xylR in low xylose concentration, the bacteria will not die. However, when the HGT of those high efficient metabolic genes happens, the death system will not be suppressed, and the recipient will be killed. | ||
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Latest revision as of 19:17, 30 September 2012
Death Device designed for probiotics
We plan to place endonuclease BglII and YhjH gene downstream the xylR repressed promoter BBa_K861200 to make bacteria die without lysis and lose their adhesion when exposed to xylose. Horizontal gene transfer can also be prevented using a two plasmid system. In one plasmid, xlyR repressor encoding gene from Bacillus subtilis will be assembled with constitutively expressed promoter. On the other plasmid, other devices can be coupled with xylR repressed death device. In our GMOs, since death system is suppressed by xylR in low xylose concentration, the bacteria will not die. However, when the HGT of those high efficient metabolic genes happens, the death system will not be suppressed, and the recipient will be killed.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]