Difference between revisions of "Part:BBa K741003"

Revision as of 17:24, 30 September 2012

pRM-RBS-GFP-T

Express GFP only if protein CI activated the promoter pRM.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 752

Figure 2 shows that the fluorescence intensity of prm-gfp is so weak in comparison with plac-gfp that we can assume that the leak expression of prm is very few, which doesn't affect our experiments.

Data

pRM-RBS-GFP-T
OD
t/min	Parallel	Experimental group	M9 medium
0	1	0.0593	0.045
	2	0.0642
	3	0.0678
45	1	0.0605	0.0436
	2	0.0734
	3	0.062
90	1	0.0648	0.0435
	2	0.0735
	3	0.0702
135	1	0.0811	0.0447
	2	0.0716
	3	0.0677
180	1	0.0723	0.0446
	2	0.0828
	3	0.0789

pRM-RBS-GFP-T
fluorescence intensity
t/min	Parallel	Experimental group	M9 medium
0	1	19.55	11.801
	2	19.571
	3	19.352
45	1	21.548	13.386
	2	20.505
	3	19.107
90	1	24.441	11.541
	2	23.777
	3	24.503
135	1	28.938	11.894
	2	27.884
	3	27.81
180	1	41.376	11.066
	2	39.573
	3	39.795

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+   width:290pt'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;pRM-RBS-GFP-T</td>
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   <tr height=19 style='height:14.25pt'>
-   <td colspan=4 height=19 class=xl26 style='height:14.25pt'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;OD</td>
+   <td colspan=4 height=19 class=xl26 style='height:14.25pt'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;OD</td>
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   </tr>
   <tr height=19 style='height:14.25pt'>
-   <td colspan=4 height=19 class=xl26 style='height:14.25pt'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;pRM-RBS-GFP-T</td>
+   <td colspan=4 height=19 class=xl26 style='height:14.25pt'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;pRM-RBS-GFP-T</td>
   </tr>
   <tr height=19 style='height:14.25pt'>
-   <td colspan=4 height=19 class=xl26 style='height:14.25pt'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;fluorescence
+   <td colspan=4 height=19 class=xl26 style='height:14.25pt'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;fluorescence
    intensity</td>
   </tr>