Difference between revisions of "Part:BBa K741003"

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[[Image:USTC2012Result2_副本.png]]
 
[[Image:USTC2012Result2_副本.png]]
 
<p>Figure 2 shows that the fluorescence intensity of prm-gfp is so weak in comparison with plac-gfp that we can assume that the leak expression of prm is very few, which doesn't affect our experiments.</p>
 
<p>Figure 2 shows that the fluorescence intensity of prm-gfp is so weak in comparison with plac-gfp that we can assume that the leak expression of prm is very few, which doesn't affect our experiments.</p>
 +
 +
==Data==
 +
 +
 +
<table x:str border=1 cellpadding=0 cellspacing=0 width=386 style='border-collapse:
 +
collapse;table-layout:fixed;width:290pt'>
 +
 +
<tr height=19 style='height:14.25pt'>
 +
  <td colspan=4 height=19 class=xl26 width=386 style='height:14.25pt;
 +
  width:290pt'>pRM-RBS-GFP-T</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td colspan=4 height=19 class=xl26 style='height:14.25pt'>OD</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl27 style='height:14.25pt;border-top:none'>t/min</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'>Parallel</td>
 +
  <td class=xl26 style='border-top:none;border-left:none'>Experimental group</td>
 +
  <td class=xl28 style='border-top:none;border-left:none'>M9 medium</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl27 style='height:14.25pt;border-top:none' x:num>0</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num>1</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'
 +
  x:num="5.9299999999999999E-2">0.0593</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'
 +
  x:num="4.4999999999999998E-2">0.045</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl27 style='height:14.25pt;border-top:none'>　</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num>2</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'
 +
  x:num="6.4199999999999993E-2">0.0642</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'>　</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl27 style='height:14.25pt;border-top:none'>　</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num>3</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'
 +
  x:num="6.7799999999999999E-2">0.0678</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'>　</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl27 style='height:14.25pt;border-top:none' x:num>45</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num>1</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'
 +
  x:num="6.0499999999999998E-2">0.0605</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num="4.36E-2">0.0436</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl27 style='height:14.25pt;border-top:none'>　</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num>2</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'
 +
  x:num="7.3400000000000007E-2">0.0734</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'>　</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl27 style='height:14.25pt;border-top:none'>　</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num>3</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num="6.2E-2">0.062</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'>　</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl27 style='height:14.25pt;border-top:none' x:num>90</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num>1</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'
 +
  x:num="6.4799999999999996E-2">0.0648</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'
 +
  x:num="4.3499999999999997E-2">0.0435</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl27 style='height:14.25pt;border-top:none'>　</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num>2</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'
 +
  x:num="7.3499999999999996E-2">0.0735</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'>　</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl27 style='height:14.25pt;border-top:none'>　</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num>3</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'
 +
  x:num="7.0199999999999999E-2">0.0702</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'>　</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl27 style='height:14.25pt;border-top:none' x:num>135</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num>1</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'
 +
  x:num="8.1100000000000005E-2">0.0811</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'
 +
  x:num="4.4699999999999997E-2">0.0447</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl27 style='height:14.25pt;border-top:none'>　</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num>2</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'
 +
  x:num="7.1599999999999997E-2">0.0716</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'>　</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl27 style='height:14.25pt;border-top:none'>　</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num>3</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'
 +
  x:num="6.7699999999999996E-2">0.0677</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'>　</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl27 style='height:14.25pt;border-top:none' x:num>180</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num>1</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'
 +
  x:num="7.2300000000000003E-2">0.0723</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'
 +
  x:num="4.4600000000000001E-2">0.0446</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl27 style='height:14.25pt;border-top:none'>　</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num>2</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'
 +
  x:num="8.2799999999999999E-2">0.0828</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'>　</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl27 style='height:14.25pt;border-top:none'>　</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num>3</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'
 +
  x:num="7.8899999999999998E-2">0.0789</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'>　</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 colspan=4 class=xl29 style='height:14.25pt;mso-ignore:colspan'></td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td colspan=4 height=19 class=xl26 style='height:14.25pt'>pRM-RBS-GFP-T</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td colspan=4 height=19 class=xl26 style='height:14.25pt'>fluorescence
 +
  intensity</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl27 style='height:14.25pt;border-top:none'>t/min</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'>Parallel</td>
 +
  <td class=xl26 style='border-top:none;border-left:none'>Experimental group</td>
 +
  <td class=xl28 style='border-top:none;border-left:none'>M9 medium</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl27 style='height:14.25pt;border-top:none' x:num>0</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num>1</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num>19.55</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num="11.801">11.801</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl27 style='height:14.25pt;border-top:none'>　</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num>2</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'
 +
  x:num="19.571000000000002">19.571</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'>　</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl27 style='height:14.25pt;border-top:none'>　</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num>3</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num="19.352">19.352</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'>　</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl27 style='height:14.25pt;border-top:none' x:num>45</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num>1</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'
 +
  x:num="21.547999999999998">21.548</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'
 +
  x:num="13.385999999999999">13.386</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl27 style='height:14.25pt;border-top:none'>　</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num>2</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'
 +
  x:num="20.504999999999999">20.505</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'>　</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl27 style='height:14.25pt;border-top:none'>　</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num>3</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'
 +
  x:num="19.106999999999999">19.107</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'>　</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl27 style='height:14.25pt;border-top:none' x:num>90</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num>1</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'
 +
  x:num="24.440999999999999">24.441</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num="11.541">11.541</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl27 style='height:14.25pt;border-top:none'>　</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num>2</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'
 +
  x:num="23.777000000000001">23.777</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'>　</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl27 style='height:14.25pt;border-top:none'>　</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num>3</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num="24.503">24.503</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'>　</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl27 style='height:14.25pt;border-top:none' x:num>135</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num>1</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'
 +
  x:num="28.937999999999999">28.938</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num="11.894">11.894</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl27 style='height:14.25pt;border-top:none'>　</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num>2</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num="27.884">27.884</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'>　</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl27 style='height:14.25pt;border-top:none'>　</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num>3</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num>27.81</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'>　</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl27 style='height:14.25pt;border-top:none' x:num>180</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num>1</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'
 +
  x:num="41.375999999999998">41.376</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'
 +
  x:num="11.066000000000001">11.066</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl27 style='height:14.25pt;border-top:none'>　</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num>2</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num="39.573">39.573</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'>　</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl27 style='height:14.25pt;border-top:none'>　</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num>3</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'
 +
  x:num="39.795000000000002">39.795</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'>　</td>
 +
</tr>
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K741003 parameters</partinfo>
 
<partinfo>BBa_K741003 parameters</partinfo>
 
<!-- -->
 
<!-- -->

Revision as of 17:21, 30 September 2012

pRM-RBS-GFP-T

Express GFP only if protein CI activated the promoter pRM.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 752

USTC2012Result2 副本.png

Figure 2 shows that the fluorescence intensity of prm-gfp is so weak in comparison with plac-gfp that we can assume that the leak expression of prm is very few, which doesn't affect our experiments.

Data

pRM-RBS-GFP-T
OD
t/min Parallel Experimental group M9 medium
0 1 0.0593 0.045
　 2 0.0642 　
　 3 0.0678 　
45 1 0.0605 0.0436
　 2 0.0734 　
　 3 0.062 　
90 1 0.0648 0.0435
　 2 0.0735 　
　 3 0.0702 　
135 1 0.0811 0.0447
　 2 0.0716 　
　 3 0.0677 　
180 1 0.0723 0.0446
　 2 0.0828 　
　 3 0.0789 　
pRM-RBS-GFP-T
fluorescence intensity
t/min Parallel Experimental group M9 medium
0 1 19.55 11.801
　 2 19.571 　
　 3 19.352 　
45 1 21.548 13.386
　 2 20.505 　
　 3 19.107 　
90 1 24.441 11.541
　 2 23.777 　
　 3 24.503 　
135 1 28.938 11.894
　 2 27.884 　
　 3 27.81 　
180 1 41.376 11.066
　 2 39.573 　
　 3 39.795