Difference between revisions of "Part:BBa K741011"
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[[Image:USTC2012Result9_副本.png]] | [[Image:USTC2012Result9_副本.png]] | ||
+ | |||
+ | ==Data== | ||
+ | |||
+ | <table x:str border=1 cellpadding=0 cellspacing=0 width=1001 style='border-collapse: | ||
+ | collapse;table-layout:fixed;width:753pt'> | ||
+ | |||
+ | <tr height=19 style='height:14.25pt'> | ||
+ | <td colspan=10 height=19 class=xl27 width=1001 style='height:14.25pt; | ||
+ | width:753pt'> plac-anticro-T-pCon(0.244)-RBS-crogfp-T</td> | ||
+ | </tr> | ||
+ | <tr height=19 style='height:14.25pt'> | ||
+ | <td colspan=10 height=19 class=xl28 style='height:14.25pt'> OD</td> | ||
+ | </tr> | ||
+ | <tr height=19 style='height:14.25pt'> | ||
+ | <td height=19 class=xl29 style='height:14.25pt;border-top:none'>t/min</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none'>Parallel</td> | ||
+ | <td class=xl30 style='border-top:none;border-left:none' x:num>0</td> | ||
+ | <td class=xl30 style='border-top:none;border-left:none'>200mM glucose</td> | ||
+ | <td class=xl30 style='border-top:none;border-left:none'>400mM glucose</td> | ||
+ | <td class=xl30 style='border-top:none;border-left:none'>600mM glucose</td> | ||
+ | <td class=xl30 style='border-top:none;border-left:none'>800mM glucose</td> | ||
+ | <td class=xl30 style='border-top:none;border-left:none'>1000mM glucose</td> | ||
+ | <td class=xl30 style='border-top:none;border-left:none'>1000mM IPTG</td> | ||
+ | <td class=xl30 style='border-top:none;border-left:none'>M9 medium</td> | ||
+ | </tr> | ||
+ | <tr height=19 style='height:14.25pt'> | ||
+ | <td height=19 class=xl29 style='height:14.25pt;border-top:none' x:num>0</td> | ||
+ | <td class=xl31 style='border-top:none;border-left:none' x:num>1</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="9.7299999999999998E-2">0.0973</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="0.1094">0.1094</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="0.10639999999999999">0.1064</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="0.111">0.111</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="0.107">0.107</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="0.1061">0.1061</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="9.7100000000000006E-2">0.0971</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="4.4200000000000003E-2">0.0442</td> | ||
+ | </tr> | ||
+ | <tr height=19 style='height:14.25pt'> | ||
+ | <td height=19 class=xl29 style='height:14.25pt;border-top:none'> </td> | ||
+ | <td class=xl31 style='border-top:none;border-left:none' x:num>2</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="9.6299999999999997E-2">0.0963</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="0.107">0.107</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="0.109">0.109</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="0.1053">0.1053</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="0.1038">0.1038</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="0.10580000000000001">0.1058</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="9.6799999999999997E-2">0.0968</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none'> </td> | ||
+ | </tr> | ||
+ | <tr height=19 style='height:14.25pt'> | ||
+ | <td height=19 class=xl29 style='height:14.25pt;border-top:none'> </td> | ||
+ | <td class=xl31 style='border-top:none;border-left:none' x:num>3</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="9.4200000000000006E-2">0.0942</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="0.1076">0.1076</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="0.1084">0.1084</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="0.1066">0.1066</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="0.1023">0.1023</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="0.10680000000000001">0.1068</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="9.7199999999999995E-2">0.0972</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none'> </td> | ||
+ | </tr> | ||
+ | <tr height=19 style='height:14.25pt'> | ||
+ | <td height=19 class=xl29 style='height:14.25pt;border-top:none' x:num>45</td> | ||
+ | <td class=xl31 style='border-top:none;border-left:none' x:num>1</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="0.1353">0.1353</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="0.1419">0.1419</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="0.15340000000000001">0.1534</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="0.16880000000000001">0.1688</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="0.1744">0.1744</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="0.17680000000000001">0.1768</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="0.12770000000000001">0.1277</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none'> </td> | ||
+ | </tr> | ||
+ | <tr height=19 style='height:14.25pt'> | ||
+ | <td height=19 class=xl29 style='height:14.25pt;border-top:none'> </td> | ||
+ | <td class=xl31 style='border-top:none;border-left:none' x:num>2</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="0.12690000000000001">0.1269</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="0.1414">0.1414</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="0.1527">0.1527</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="0.1714">0.1714</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="0.1918">0.1918</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="0.17560000000000001">0.1756</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="0.1313">0.1313</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none'> </td> | ||
+ | </tr> | ||
+ | <tr height=19 style='height:14.25pt'> | ||
+ | <td height=19 class=xl29 style='height:14.25pt;border-top:none'> </td> | ||
+ | <td class=xl31 style='border-top:none;border-left:none' x:num>3</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="0.12859999999999999">0.1286</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="0.13869999999999999">0.1387</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="0.14810000000000001">0.1481</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="0.16819999999999999">0.1682</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="0.17760000000000001">0.1776</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="0.1744">0.1744</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="0.12479999999999999">0.1248</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="4.1599999999999998E-2">0.0416</td> | ||
+ | </tr> | ||
+ | <tr height=19 style='height:14.25pt'> | ||
+ | <td height=19 class=xl29 style='height:14.25pt;border-top:none' x:num>90</td> | ||
+ | <td class=xl31 style='border-top:none;border-left:none' x:num>1</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="0.16689999999999999">0.1669</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="0.1797">0.1797</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="0.18410000000000001">0.1841</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num>0.2</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="0.2051">0.2051</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="0.19450000000000001">0.1945</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num>0.18</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none'> </td> | ||
+ | </tr> | ||
+ | <tr height=19 style='height:14.25pt'> | ||
+ | <td height=19 class=xl29 style='height:14.25pt;border-top:none'> </td> | ||
+ | <td class=xl31 style='border-top:none;border-left:none' x:num>2</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="0.1663">0.1663</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="0.1741">0.1741</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="0.18360000000000001">0.1836</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="0.19689999999999999">0.1969</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="0.19409999999999999">0.1941</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="0.20880000000000001">0.2088</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="0.1663">0.1663</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none'> </td> | ||
+ | </tr> | ||
+ | <tr height=19 style='height:14.25pt'> | ||
+ | <td height=19 class=xl29 style='height:14.25pt;border-top:none'> </td> | ||
+ | <td class=xl31 style='border-top:none;border-left:none' x:num>3</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="0.1653">0.1653</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="0.17610000000000001">0.1761</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="0.1847">0.1847</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="0.19620000000000001">0.1962</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="0.20230000000000001">0.2023</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="0.19500000000000001">0.195</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="0.17699999999999999">0.177</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="4.02E-2">0.0402</td> | ||
+ | </tr> | ||
+ | <tr height=19 style='height:14.25pt'> | ||
+ | <td height=19 class=xl29 style='height:14.25pt;border-top:none' x:num>135</td> | ||
+ | <td class=xl31 style='border-top:none;border-left:none' x:num>1</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="0.2079">0.2079</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="0.20849999999999999">0.2085</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="0.2109">0.2109</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="0.22789999999999999">0.2279</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="0.22720000000000001">0.2272</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="0.2072">0.2072</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="0.2097">0.2097</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none'> </td> | ||
+ | </tr> | ||
+ | <tr height=19 style='height:14.25pt'> | ||
+ | <td height=19 class=xl29 style='height:14.25pt;border-top:none'> </td> | ||
+ | <td class=xl31 style='border-top:none;border-left:none' x:num>2</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="0.21440000000000001">0.2144</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="0.20380000000000001">0.2038</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="0.21579999999999999">0.2158</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="0.2104">0.2104</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="0.19919999999999999">0.1992</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="0.20680000000000001">0.2068</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="0.20519999999999999">0.2052</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none'> </td> | ||
+ | </tr> | ||
+ | <tr height=19 style='height:14.25pt'> | ||
+ | <td height=19 class=xl29 style='height:14.25pt;border-top:none'> </td> | ||
+ | <td class=xl31 style='border-top:none;border-left:none' x:num>3</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="0.2107">0.2107</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="0.20380000000000001">0.2038</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="0.2031">0.2031</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="0.22850000000000001">0.2285</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="0.22500000000000001">0.225</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="0.19620000000000001">0.1962</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="0.2034">0.2034</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="4.1099999999999998E-2">0.0411</td> | ||
+ | </tr> | ||
+ | <tr height=19 style='height:14.25pt'> | ||
+ | <td height=19 class=xl29 style='height:14.25pt;border-top:none' x:num>180</td> | ||
+ | <td class=xl31 style='border-top:none;border-left:none' x:num>1</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="0.22140000000000001">0.2214</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="0.2137">0.2137</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="0.21590000000000001">0.2159</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="0.2331">0.2331</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="0.2414">0.2414</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="0.2114">0.2114</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="0.2132">0.2132</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none'> </td> | ||
+ | </tr> | ||
+ | <tr height=19 style='height:14.25pt'> | ||
+ | <td height=19 class=xl29 style='height:14.25pt;border-top:none'> </td> | ||
+ | <td class=xl31 style='border-top:none;border-left:none' x:num>2</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="0.215">0.215</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="0.20480000000000001">0.2048</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="0.22120000000000001">0.2212</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="0.21790000000000001">0.2179</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="0.20810000000000001">0.2081</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="0.20749999999999999">0.2075</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="0.20680000000000001">0.2068</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none'> </td> | ||
+ | </tr> | ||
+ | <tr height=19 style='height:14.25pt'> | ||
+ | <td height=19 class=xl29 style='height:14.25pt;border-top:none'> </td> | ||
+ | <td class=xl31 style='border-top:none;border-left:none' x:num>3</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="0.2132">0.2132</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="0.21179999999999999">0.2118</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="0.21640000000000001">0.2164</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="0.23250000000000001">0.2325</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="0.2316">0.2316</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="0.19889999999999999">0.1989</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="0.2074">0.2074</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="4.19E-2">0.0419</td> | ||
+ | </tr> | ||
+ | <tr height=19 style='height:14.25pt'> | ||
+ | <td height=19 class=xl29 style='height:14.25pt;border-top:none' x:num>225</td> | ||
+ | <td class=xl31 style='border-top:none;border-left:none' x:num>1</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="0.22520000000000001">0.2252</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="0.2117">0.2117</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="0.20930000000000001">0.2093</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="0.2339">0.2339</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="0.22939999999999999">0.2294</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="0.2082">0.2082</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="0.21659999999999999">0.2166</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none'> </td> | ||
+ | </tr> | ||
+ | <tr height=19 style='height:14.25pt'> | ||
+ | <td height=19 class=xl29 style='height:14.25pt;border-top:none'> </td> | ||
+ | <td class=xl31 style='border-top:none;border-left:none' x:num>2</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="0.21590000000000001">0.2159</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="0.20699999999999999">0.207</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="0.22059999999999999">0.2206</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="0.21729999999999999">0.2173</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="0.20710000000000001">0.2071</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="0.21129999999999999">0.2113</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="0.2082">0.2082</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none'> </td> | ||
+ | </tr> | ||
+ | <tr height=19 style='height:14.25pt'> | ||
+ | <td height=19 class=xl29 style='height:14.25pt;border-top:none'> </td> | ||
+ | <td class=xl31 style='border-top:none;border-left:none' x:num>3</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="0.21329999999999999">0.2133</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="0.21249999999999999">0.2125</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="0.21779999999999999">0.2178</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="0.24010000000000001">0.2401</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="0.23130000000000001">0.2313</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="0.2009">0.2009</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="0.2104">0.2104</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="3.9600000000000003E-2">0.0396</td> | ||
+ | </tr> | ||
+ | <tr height=19 style='height:14.25pt'> | ||
+ | <td height=19 colspan=10 class=xl32 style='height:14.25pt;mso-ignore:colspan'></td> | ||
+ | </tr> | ||
+ | <tr height=19 style='height:14.25pt'> | ||
+ | <td colspan=10 height=19 class=xl27 style='height:14.25pt'> plac-anticro-T-pCon(0.244)-RBS-crogfp-T</td> | ||
+ | </tr> | ||
+ | <tr height=19 style='height:14.25pt'> | ||
+ | <td colspan=10 height=19 class=xl28 style='height:14.25pt'> Fluorescence | ||
+ | intensity</td> | ||
+ | </tr> | ||
+ | <tr height=19 style='height:14.25pt'> | ||
+ | <td height=19 class=xl29 style='height:14.25pt;border-top:none'>t/min</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none'>Parallel</td> | ||
+ | <td class=xl30 style='border-top:none;border-left:none' x:num>0</td> | ||
+ | <td class=xl30 style='border-top:none;border-left:none'>200mM glucose</td> | ||
+ | <td class=xl30 style='border-top:none;border-left:none'>400mM glucose</td> | ||
+ | <td class=xl30 style='border-top:none;border-left:none'>600mM glucose</td> | ||
+ | <td class=xl30 style='border-top:none;border-left:none'>800mM glucose</td> | ||
+ | <td class=xl30 style='border-top:none;border-left:none'>1000mM glucose</td> | ||
+ | <td class=xl30 style='border-top:none;border-left:none'>1000mM IPTG</td> | ||
+ | <td class=xl30 style='border-top:none;border-left:none'>M9 medium</td> | ||
+ | </tr> | ||
+ | <tr height=19 style='height:14.25pt'> | ||
+ | <td height=19 class=xl29 style='height:14.25pt;border-top:none' x:num>0</td> | ||
+ | <td class=xl31 style='border-top:none;border-left:none' x:num>1</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="81.831999999999994">81.832</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="83.433999999999997">83.434</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num>86.34</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="85.412999999999997">85.413</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="75.242000000000004">75.242</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="79.313999999999993">79.314</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="80.353999999999999">80.354</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="14.031000000000001">14.031</td> | ||
+ | </tr> | ||
+ | <tr height=19 style='height:14.25pt'> | ||
+ | <td height=19 class=xl29 style='height:14.25pt;border-top:none'> </td> | ||
+ | <td class=xl31 style='border-top:none;border-left:none' x:num>2</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="78.831999999999994">78.832</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num>83.66</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="83.361999999999995">83.362</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="85.971000000000004">85.971</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="80.54">80.54</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="81.980999999999995">81.981</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="80.692999999999998">80.693</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none'> </td> | ||
+ | </tr> | ||
+ | <tr height=19 style='height:14.25pt'> | ||
+ | <td height=19 class=xl29 style='height:14.25pt;border-top:none'> </td> | ||
+ | <td class=xl31 style='border-top:none;border-left:none' x:num>3</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num>83.71</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="79.356999999999999">79.357</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="85.259">85.259</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="80.691999999999993">80.692</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="78.863">78.863</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num>75.19</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="77.573999999999998">77.574</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none'> </td> | ||
+ | </tr> | ||
+ | <tr height=19 style='height:14.25pt'> | ||
+ | <td height=19 class=xl29 style='height:14.25pt;border-top:none' x:num>45</td> | ||
+ | <td class=xl31 style='border-top:none;border-left:none' x:num>1</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="97.073999999999998">97.074</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="96.549000000000007">96.549</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="105.152">105.152</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="96.747">96.747</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="109.125">109.125</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="113.18899999999999">113.189</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="104.506">104.506</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none'> </td> | ||
+ | </tr> | ||
+ | <tr height=19 style='height:14.25pt'> | ||
+ | <td height=19 class=xl29 style='height:14.25pt;border-top:none'> </td> | ||
+ | <td class=xl31 style='border-top:none;border-left:none' x:num>2</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="105.45399999999999">105.454</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="102.764">102.764</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="110.095">110.095</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="106.93899999999999">106.939</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="101.639">101.639</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="105.917">105.917</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="102.98699999999999">102.987</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none'> </td> | ||
+ | </tr> | ||
+ | <tr height=19 style='height:14.25pt'> | ||
+ | <td height=19 class=xl29 style='height:14.25pt;border-top:none'> </td> | ||
+ | <td class=xl31 style='border-top:none;border-left:none' x:num>3</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num>91.59</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="101.685">101.685</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="112.482">112.482</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="109.825">109.825</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="120.504">120.504</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="102.764">102.764</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num>117.71</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="14.037000000000001">14.037</td> | ||
+ | </tr> | ||
+ | <tr height=19 style='height:14.25pt'> | ||
+ | <td height=19 class=xl29 style='height:14.25pt;border-top:none' x:num>90</td> | ||
+ | <td class=xl31 style='border-top:none;border-left:none' x:num>1</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="114.849">114.849</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="120.274">120.274</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="128.54400000000001">128.544</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num>130.12</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="133.07900000000001">133.079</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="136.33799999999999">136.338</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="116.663">116.663</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none'> </td> | ||
+ | </tr> | ||
+ | <tr height=19 style='height:14.25pt'> | ||
+ | <td height=19 class=xl29 style='height:14.25pt;border-top:none'> </td> | ||
+ | <td class=xl31 style='border-top:none;border-left:none' x:num>2</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="117.428">117.428</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="124.116">124.116</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="114.105">114.105</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="137.70099999999999">137.701</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="134.494">134.494</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="143.047">143.047</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="119.459">119.459</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none'> </td> | ||
+ | </tr> | ||
+ | <tr height=19 style='height:14.25pt'> | ||
+ | <td height=19 class=xl29 style='height:14.25pt;border-top:none'> </td> | ||
+ | <td class=xl31 style='border-top:none;border-left:none' x:num>3</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="115.149">115.149</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="114.123">114.123</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="131.119">131.119</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="128.50800000000001">128.508</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="136.20500000000001">136.205</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="138.58">138.58</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="119.333">119.333</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="14.013">14.013</td> | ||
+ | </tr> | ||
+ | <tr height=19 style='height:14.25pt'> | ||
+ | <td height=19 class=xl29 style='height:14.25pt;border-top:none' x:num>135</td> | ||
+ | <td class=xl31 style='border-top:none;border-left:none' x:num>1</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="149.328">149.328</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="130.73">130.73</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="149.45699999999999">149.457</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="138.964">138.964</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="161.75299999999999">161.753</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="156.79400000000001">156.794</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num>136.9</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none'> </td> | ||
+ | </tr> | ||
+ | <tr height=19 style='height:14.25pt'> | ||
+ | <td height=19 class=xl29 style='height:14.25pt;border-top:none'> </td> | ||
+ | <td class=xl31 style='border-top:none;border-left:none' x:num>2</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="139.52199999999999">139.522</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="133.45400000000001">133.454</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="145.12100000000001">145.121</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="158.92400000000001">158.924</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="174.357">174.357</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="157.161">157.161</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="144.88200000000001">144.882</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none'> </td> | ||
+ | </tr> | ||
+ | <tr height=19 style='height:14.25pt'> | ||
+ | <td height=19 class=xl29 style='height:14.25pt;border-top:none'> </td> | ||
+ | <td class=xl31 style='border-top:none;border-left:none' x:num>3</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="129.327">129.327</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="141.83600000000001">141.836</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="146.58699999999999">146.587</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="149.863">149.863</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="156.21100000000001">156.211</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="146.249">146.249</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num>140.91</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="14.449">14.449</td> | ||
+ | </tr> | ||
+ | <tr height=19 style='height:14.25pt'> | ||
+ | <td height=19 class=xl29 style='height:14.25pt;border-top:none' x:num>180</td> | ||
+ | <td class=xl31 style='border-top:none;border-left:none' x:num>1</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="155.74299999999999">155.743</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="167.42099999999999">167.421</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="175.02699999999999">175.027</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="164.577">164.577</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="190.285">190.285</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="162.935">162.935</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="141.19800000000001">141.198</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none'> </td> | ||
+ | </tr> | ||
+ | <tr height=19 style='height:14.25pt'> | ||
+ | <td height=19 class=xl29 style='height:14.25pt;border-top:none'> </td> | ||
+ | <td class=xl31 style='border-top:none;border-left:none' x:num>2</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="166.68600000000001">166.686</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="166.27199999999999">166.272</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="168.98699999999999">168.987</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="177.399">177.399</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="170.245">170.245</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="142.566">142.566</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="148.96100000000001">148.961</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none'> </td> | ||
+ | </tr> | ||
+ | <tr height=19 style='height:14.25pt'> | ||
+ | <td height=19 class=xl29 style='height:14.25pt;border-top:none'> </td> | ||
+ | <td class=xl31 style='border-top:none;border-left:none' x:num>3</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="168.15600000000001">168.156</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="163.31100000000001">163.311</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="172.91399999999999">172.914</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="182.47300000000001">182.473</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="164.398">164.398</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="145.16499999999999">145.165</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="148.66300000000001">148.663</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="13.826000000000001">13.826</td> | ||
+ | </tr> | ||
+ | <tr height=19 style='height:14.25pt'> | ||
+ | <td height=19 class=xl29 style='height:14.25pt;border-top:none' x:num>225</td> | ||
+ | <td class=xl31 style='border-top:none;border-left:none' x:num>1</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num>155.82</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="164.911">164.911</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="176.17099999999999">176.171</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="188.184">188.184</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="185.86500000000001">185.865</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="163.27799999999999">163.278</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="163.715">163.715</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none'> </td> | ||
+ | </tr> | ||
+ | <tr height=19 style='height:14.25pt'> | ||
+ | <td height=19 class=xl29 style='height:14.25pt;border-top:none'> </td> | ||
+ | <td class=xl31 style='border-top:none;border-left:none' x:num>2</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="172.05600000000001">172.056</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="160.626">160.626</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="169.286">169.286</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="186.381">186.381</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="169.715">169.715</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="172.464">172.464</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="158.55099999999999">158.551</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none'> </td> | ||
+ | </tr> | ||
+ | <tr height=19 style='height:14.25pt'> | ||
+ | <td height=19 class=xl29 style='height:14.25pt;border-top:none'> </td> | ||
+ | <td class=xl31 style='border-top:none;border-left:none' x:num>3</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="171.75800000000001">171.758</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="172.44200000000001">172.442</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="181.38200000000001">181.382</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' x:num="192.399">192.399</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="185.04900000000001">185.049</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="156.61600000000001">156.616</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="159.47300000000001">159.473</td> | ||
+ | <td class=xl29 style='border-top:none;border-left:none' | ||
+ | x:num="14.122999999999999">14.123</td> | ||
+ | </tr> | ||
<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display | ||
===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_K741011 parameters</partinfo> | <partinfo>BBa_K741011 parameters</partinfo> | ||
<!-- --> | <!-- --> |
Revision as of 17:15, 30 September 2012
plac-anticro-T-pCon(0.244)-RBS-crogfp-T
The fusion protein crogfp is constantly expressed and the antisense RNA of cro is produced to inhibit the translation of the mRNA of crogfp after IPTG activated the promoter plac.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 124
Illegal BglII site found at 1481 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 918
Data
plac-anticro-T-pCon(0.244)-RBS-crogfp-T | |||||||||
OD | |||||||||
t/min | Parallel | 0 | 200mM glucose | 400mM glucose | 600mM glucose | 800mM glucose | 1000mM glucose | 1000mM IPTG | M9 medium |
0 | 1 | 0.0973 | 0.1094 | 0.1064 | 0.111 | 0.107 | 0.1061 | 0.0971 | 0.0442 |
2 | 0.0963 | 0.107 | 0.109 | 0.1053 | 0.1038 | 0.1058 | 0.0968 | ||
3 | 0.0942 | 0.1076 | 0.1084 | 0.1066 | 0.1023 | 0.1068 | 0.0972 | ||
45 | 1 | 0.1353 | 0.1419 | 0.1534 | 0.1688 | 0.1744 | 0.1768 | 0.1277 | |
2 | 0.1269 | 0.1414 | 0.1527 | 0.1714 | 0.1918 | 0.1756 | 0.1313 | ||
3 | 0.1286 | 0.1387 | 0.1481 | 0.1682 | 0.1776 | 0.1744 | 0.1248 | 0.0416 | |
90 | 1 | 0.1669 | 0.1797 | 0.1841 | 0.2 | 0.2051 | 0.1945 | 0.18 | |
2 | 0.1663 | 0.1741 | 0.1836 | 0.1969 | 0.1941 | 0.2088 | 0.1663 | ||
3 | 0.1653 | 0.1761 | 0.1847 | 0.1962 | 0.2023 | 0.195 | 0.177 | 0.0402 | |
135 | 1 | 0.2079 | 0.2085 | 0.2109 | 0.2279 | 0.2272 | 0.2072 | 0.2097 | |
2 | 0.2144 | 0.2038 | 0.2158 | 0.2104 | 0.1992 | 0.2068 | 0.2052 | ||
3 | 0.2107 | 0.2038 | 0.2031 | 0.2285 | 0.225 | 0.1962 | 0.2034 | 0.0411 | |
180 | 1 | 0.2214 | 0.2137 | 0.2159 | 0.2331 | 0.2414 | 0.2114 | 0.2132 | |
2 | 0.215 | 0.2048 | 0.2212 | 0.2179 | 0.2081 | 0.2075 | 0.2068 | ||
3 | 0.2132 | 0.2118 | 0.2164 | 0.2325 | 0.2316 | 0.1989 | 0.2074 | 0.0419 | |
225 | 1 | 0.2252 | 0.2117 | 0.2093 | 0.2339 | 0.2294 | 0.2082 | 0.2166 | |
2 | 0.2159 | 0.207 | 0.2206 | 0.2173 | 0.2071 | 0.2113 | 0.2082 | ||
3 | 0.2133 | 0.2125 | 0.2178 | 0.2401 | 0.2313 | 0.2009 | 0.2104 | 0.0396 | |
plac-anticro-T-pCon(0.244)-RBS-crogfp-T | |||||||||
Fluorescence intensity | |||||||||
t/min | Parallel | 0 | 200mM glucose | 400mM glucose | 600mM glucose | 800mM glucose | 1000mM glucose | 1000mM IPTG | M9 medium |
0 | 1 | 81.832 | 83.434 | 86.34 | 85.413 | 75.242 | 79.314 | 80.354 | 14.031 |
2 | 78.832 | 83.66 | 83.362 | 85.971 | 80.54 | 81.981 | 80.693 | ||
3 | 83.71 | 79.357 | 85.259 | 80.692 | 78.863 | 75.19 | 77.574 | ||
45 | 1 | 97.074 | 96.549 | 105.152 | 96.747 | 109.125 | 113.189 | 104.506 | |
2 | 105.454 | 102.764 | 110.095 | 106.939 | 101.639 | 105.917 | 102.987 | ||
3 | 91.59 | 101.685 | 112.482 | 109.825 | 120.504 | 102.764 | 117.71 | 14.037 | |
90 | 1 | 114.849 | 120.274 | 128.544 | 130.12 | 133.079 | 136.338 | 116.663 | |
2 | 117.428 | 124.116 | 114.105 | 137.701 | 134.494 | 143.047 | 119.459 | ||
3 | 115.149 | 114.123 | 131.119 | 128.508 | 136.205 | 138.58 | 119.333 | 14.013 | |
135 | 1 | 149.328 | 130.73 | 149.457 | 138.964 | 161.753 | 156.794 | 136.9 | |
2 | 139.522 | 133.454 | 145.121 | 158.924 | 174.357 | 157.161 | 144.882 | ||
3 | 129.327 | 141.836 | 146.587 | 149.863 | 156.211 | 146.249 | 140.91 | 14.449 | |
180 | 1 | 155.743 | 167.421 | 175.027 | 164.577 | 190.285 | 162.935 | 141.198 | |
2 | 166.686 | 166.272 | 168.987 | 177.399 | 170.245 | 142.566 | 148.961 | ||
3 | 168.156 | 163.311 | 172.914 | 182.473 | 164.398 | 145.165 | 148.663 | 13.826 | |
225 | 1 | 155.82 | 164.911 | 176.171 | 188.184 | 185.865 | 163.278 | 163.715 | |
2 | 172.056 | 160.626 | 169.286 | 186.381 | 169.715 | 172.464 | 158.551 | ||
3 | 171.758 | 172.442 | 181.382 | 192.399 | 185.049 | 156.616 | 159.473 | 14.123 |