Difference between revisions of "Part:BBa K845005"
Greghansen (Talk | contribs) |
|||
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K845005 short</partinfo> | <partinfo>BBa_K845005 short</partinfo> | ||
| − | ... | + | In order to detect the S.Aureus we take inspiration on the parasite project |
| + | ( http://2010.igem.org/Team:Imperial_College_London ) but there was one problem there is no peptide detector in E.coli who was able to activate a promoter. One receptor, involved in chemotaxis, detects few di/tri peptides (Tap receptor). In order to activate a promoter in presence of that peptide we designed a hybrid Tap/EnvZ receptor (which we called TapZ). | ||
| + | EnvZ is a receptor involved in osmotic pressure sensing. It phosphorylates ompR which activates the pompC promoter. | ||
| + | We must use this part in a BW25113 ΔenvZ. | ||
| + | |||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 17:00, 30 September 2012
TapZ engineered membrane receptor
In order to detect the S.Aureus we take inspiration on the parasite project ( http://2010.igem.org/Team:Imperial_College_London ) but there was one problem there is no peptide detector in E.coli who was able to activate a promoter. One receptor, involved in chemotaxis, detects few di/tri peptides (Tap receptor). In order to activate a promoter in presence of that peptide we designed a hybrid Tap/EnvZ receptor (which we called TapZ). EnvZ is a receptor involved in osmotic pressure sensing. It phosphorylates ompR which activates the pompC promoter. We must use this part in a BW25113 ΔenvZ.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]