Difference between revisions of "Part:BBa K812120"

Latest revision as of 16:58, 30 September 2012

IaaH on PSC2+ Ready for use in xenopus tropicalis egg

*** WARNING : THIS PART HAS BEEN MERGED WITH BBa_K812020. IT IS AVAILABLE IN pSB1C3 AND IN FROG-READY PLASMID BACKBONES BBa_K812000.***

Corresponds to : This part contains the coding sequence for Indoleacetamide hydrolyase (IaaH). This part is from BBa_K515100, but it was modified to increase the expression in eukaryotic cells by adding a Kozak sequence in front of the original sequence.

This part have the plasmid backbone BBa_K8120000 and the part J04450 is replaced by IaaH BBa_K812000.

This plasmid is ready for injection in tadpole.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1025
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K812120 short</partinfo>
+<html>
+</br>
+<b>*** WARNING : THIS PART HAS BEEN MERGED WITH <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812020">BBa_K812020</a>. IT IS AVAILABLE IN pSB1C3 AND IN FROG-READY PLASMID BACKBONES <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812000">BBa_K812000</a>.***</b>
+</br></br>
 Corresponds to :
-<html>
 This part contains the coding sequence for Indoleacetamide hydrolyase (IaaH). This part is from  <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K515100">BBa_K515100</a>, but it was modified to increase the expression in eukaryotic cells by adding a Kozak sequence in front of the original sequence.</br></br>