Difference between revisions of "Part:BBa K812331"
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<b>*** WARNING : THIS PART HAS BEEN MERGED WITH <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812031">BBa_K812031</a>. IT IS AVAILABLE IN pSB1C3 AND IN FROG-READY PLASMID BACKBONES <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812300">BBa_K812300</a> (HSP70 promoter).***</b>
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<b>*** WARNING : THIS PART HAS BEEN MERGED WITH <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812031">BBa_K812031</a>. IT IS AVAILABLE IN pSB1C3 AND IN FROG-READY PLASMID BACKBONES <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812000">BBa_K812000</a> (CMV promoter), <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812300">BBa_K812300</a> (HSP70 promoter), <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812200">BBa_K812200</a> (elastase promoter).***</b>
 
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Revision as of 16:49, 30 September 2012

sfGFP in pCS2+ with HSP70 promoter



*** WARNING : THIS PART HAS BEEN MERGED WITH BBa_K812031. IT IS AVAILABLE IN pSB1C3 AND IN FROG-READY PLASMID BACKBONES BBa_K812000 (CMV promoter), BBa_K812300 (HSP70 promoter), BBa_K812200 (elastase promoter).***

Super folded Green fluorescent protein (sfGFP) fused with a kozak sequence to enhance its expression in eukaryotic cells. This part is in pCS2+, the plasmid backbone is BBa_K812300
This part was created with the plasmid backbone BBa_K812300: J04450 was replaced by sfGFP (BBa_K812031) and the CMV promoter was replaced by the Hsp70 (Heat Shock Promoter) promoter promoter with SalI and HindIII restriction sites from BBa_K812300.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 19