Difference between revisions of "Part:BBa K741008"

Revision as of 16:26, 30 September 2012

pCon(0.244)-RBS-crogfp-T

Here, pCon(J23114) constantly expressing the fusion protein crogfp at 10% of the strength of pCon(J23100).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 124
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 918

Figure 5 shows the difference in intensity of expression of pCon(0.244) and pCon(0.856). Obviously pCon(0.856) expresses much stronger than pCon(0.244).

Data

pCon(0.244)-RBS-crogfp-T
	OD			fluorescence intensity
t/min	Parellel	Experimental group	M9 medium	Parellel	Experimental group	M9 medium
0	1	0.136	0.0559	1	132.355	18.095
	2	0.1313		2	116.709
45	1	0.1988	0.0522	1	180.537	19.878
	2	0.2004		2	172.497
90	1	0.3153	0.0543	1	267.399	21.225
	2	0.3126		2	243.579
135	1	0.445	0.0541	1	346.814	20.907
	2	0.4478		2	357.696
180	1	0.4949	0.0533	1	423.673	21.545
	2	0.5014		2	454.136

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    <td colspan=3 class=xl24 style='border-left:none'>OD</td>
-   <td colspan=3 class=xl24 style='border-left:none'>fluorescence intensity</td>
+   <td colspan=3 class=xl24 style='border-left:none'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;fluorescence intensity</td>
   </tr>
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