Difference between revisions of "Part:BBa K741001"

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   height:14.25pt;width:918pt; align:center;'>Fluorescence intensity</td>
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   height:14.25pt;width:918pt;'align="center">Fluorescence intensity</td>
 
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   height:14.25pt'>plac-RBS-GFP-T</td>
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   height:14.25pt'align="center">plac-RBS-GFP-T</td>
 
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Revision as of 15:24, 30 September 2012

plac-RBS-cI-T

It can generates protein CI whereas the expression can only be started when the promoter plac is activated by IPTG.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Data

Fluorescence intensity
plac-RBS-GFP-T
t/min M9 medium 0 500mM glucose 800mM glucose 1000mM glucose 1000mM IPTG
Parallel 1 1 2 3 1 2 3 1 2 3 1 2 3 1 2 3
0 11.801 197.047 198.876 190.953 202.614 203.782 202.702 200.39 202.035 204.525 206.516 206.355 206.741 179.988 210.315 205.464
45 13.386 214.145 213.602 219.416 223.437 241.267 224.909 244.257 235.427 243.904 224.783 235.979 239.014 209.009 214.669 224.21
90 11.541 273.664 245.629 250.211 305.733 317.547 313.495 299.869 324.396 328.157 331.194 309.167 305.24 261.801 267.369 281.328
135 11.894 344.987 319.854 325.421 370.167 398.352 369.309 372.487 407.478 385.073 333.989 355.2 351.929 314.246 332.2 351.364
180 11.066 424.182 425.564 389.785 415.844 463.184 378.606 447.508 456.27 449.414 311.226 441.902 456.969 338.101 304.146 349.339