Difference between revisions of "Part:BBa K845001"
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Revision as of 13:36, 30 September 2012
New paraBAD part
iGEM 2012 Grenoble Team proposes a new design and application to the pBAD promoter(paraBAD).
In order to create a biological AND gate the 2012 Grenoble team worked on the cAMP-CRP complex and arabinose inductible promoter.
Usage and Biology
paraBAD is a promoter which can be activated by 2 molecules : CRP-cAMP complex and the AraC protein. It has two states; in absence of L-arabinose the paraBAD promoter is repressed by AraC, whereas the paraC promoter is activated (unless an excess of AraC is present).
In presence of L-arabinose and the CRP-cAMP complex, the promoter is activated thus enabling the transcription of the downstream elements.
This AND gate provides a filter to biological noise.
ecocyc.org (Transcription Unit: araBAD)
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 344
Illegal XhoI site found at 494 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 179
Illegal AgeI site found at 484 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 161