Difference between revisions of "Part:BBa K741018"
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K741018 SequenceAndFeatures</partinfo> | <partinfo>BBa_K741018 SequenceAndFeatures</partinfo> | ||
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| − | + | <p>Figure 7. shows similar effects of glucose and IPTG on plac-cI-pRM-anticroT-pcon(0.856)-crogfp. pRM is activated by the protein cI. By adding IPTG, plac is activated, which leads to the production of the protein cI. With the existence of cI, pRM-anticroT starts to work and antirco represses the translation of crogfp. On the contrast, glucose represses the expression of plac, which reduces the protein cI. pRM expresses less and the translation of crogfp is repressed less. Therefore, the unit fluorescence intensity of experimental groups with glucose added is the highest.</p> | |
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===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_K741018 parameters</partinfo> | <partinfo>BBa_K741018 parameters</partinfo> | ||
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Revision as of 08:27, 30 September 2012
plac-RBS-cI-T-pRM-anticro-T-pCon(0.856)-RBS-crogfp-T
The fusion protein crogfp downstream the constitutive promoter BBa_J23102 is continuously expressed. If IPTG exists, the cI will be expressed and then activates the pRM to produce the antisense RNA of cro(anticro). The anticro will repress the expression of crogfp.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1589
Illegal NheI site found at 1612 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1370
Illegal BglII site found at 1706 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2500
Figure 7. shows similar effects of glucose and IPTG on plac-cI-pRM-anticroT-pcon(0.856)-crogfp. pRM is activated by the protein cI. By adding IPTG, plac is activated, which leads to the production of the protein cI. With the existence of cI, pRM-anticroT starts to work and antirco represses the translation of crogfp. On the contrast, glucose represses the expression of plac, which reduces the protein cI. pRM expresses less and the translation of crogfp is repressed less. Therefore, the unit fluorescence intensity of experimental groups with glucose added is the highest.