Difference between revisions of "Part:BBa K864401"

(→‎Source)
(→‎Source)
Line 5: Line 5:
  
 
===Source===
 
===Source===
−
The protein was first extracted and characterized in (Shkrob et. al., 2005) under the name aeCP597. This version is codon optimized for E.''coli''
+
The protein was first extracted and characterized by Shkrob et. al. 2005 under the name aeCP597. This version is codon optimized for '' E coli'' by Bioneer Corp.
  
−
Shkrob et. al. reported that the sum of the following substitutions compared to wild-type resulted in a far-red-shifted but radiation sensitive fluorescent protein: (K6T, K7E. Y101S, C143S, M146I, S158A)
+
Below to the left is the expressed aeBlue in ''E coli''. To the right comparison of aeBlue with other chromoproteins available from the registry.
  
−
Adding these following substitutions created a more thermostable and photostable product than the above product: (S3P, V19I, Y101S, M146I, N168D)
 
−
 
−
Below to the left is the expressed aeBlue in ''E.coli''. To the right comparison of aeBlue with other chromoproteins.
 
 
[[Image:Aeblue_plate_small.jpg.png|360px]]
 
[[Image:Aeblue_plate_small.jpg.png|360px]]
 
[[Image:UUChromo.jpg|500px]]
 
[[Image:UUChromo.jpg|500px]]

Revision as of 00:36, 30 September 2012

aeBlue blue chromoprotein

aeBlue is a blue chromoprotein extracted from the basal disk of a beadlet anemone Actinia equina. The protein has an absorption maximum at 597nm and a deep blue colour. The protein aeBlue has significant sequence homologies with proteins in the GFP family.

Source

The protein was first extracted and characterized by Shkrob et. al. 2005 under the name aeCP597. This version is codon optimized for E coli by Bioneer Corp.

Below to the left is the expressed aeBlue in E coli. To the right comparison of aeBlue with other chromoproteins available from the registry.

Aeblue plate small.jpg.png UUChromo.jpg

References

[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1316306/]Shkrob, M.A., Yanushevich, Y.G., Chudakov, D.M., Gurskaya, N.G., Labas, Y.A., Poponov, S.Y., Mudrik, N.N., Lukyanov, S., Lukyanov, K.A., 2005. Far-red fluorescent proteins evolved from a blue chromoprotein from Actinia equina. Biochem. J. 392, 649–654.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]