Difference between revisions of "Part:BBa K864050:Design"

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===References===
 
===References===
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[http://dx.doi.org/10.1016/j.gene.2006.04.018] Datta, S., Costantino, N. & Court, D.L. A set of recombineering plasmids for gram-negative bacteria. Gene 379, 109–115 (2006).

Revision as of 23:50, 29 September 2012

pSC101 low copy origin of replication


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2130
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This ori was created by cloning pSC101ts from pSIM5 and making a V56A mutation in repA.


Source

The pSC101ts origin was cloned from the pSIM5 plasmid from Datta et al, see reference below. One point mutation was made to remove one illegal SpeI site.

References

[http://dx.doi.org/10.1016/j.gene.2006.04.018] Datta, S., Costantino, N. & Court, D.L. A set of recombineering plasmids for gram-negative bacteria. Gene 379, 109–115 (2006).