Difference between revisions of "Talk:Part:BBa J45004"
 
Line 14: Line 14:
 
#Down-regulated "in planta" by ethylene (regulated spatially and temporally)
 
#Down-regulated "in planta" by ethylene (regulated spatially and temporally)
 
#References & Links
 
#References & Links
−
[http://openwetware.org/wiki/IGEM:MIT/2006/Notebook/2006-6-6 link OWW Wiki]
+
[http://openwetware.org/wiki/IGEM:MIT/2006/Notebook/2006-6-6 OWW Wiki]

Latest revision as of 20:52, 7 June 2006

  1. Species: Petunia x hybrida, A. thaliana and N. suaveolens
  2. Sequence & Structure
    • Screen cDNA-> subcloned into the NdeI-EcoRI site of the expression vector pET-28a; E. coli
    • Purified/Washed afterwards
    • Material availability: cDNA from Petunia x hybrida- phBSMT1, phBSMT2; (sequence not available? from A. thaliana)
  3. Reaction catalyzed
    • Substrate: benzoic acid or salicylic acid supplied exogenously
    • Produces methyl benzoate/methyl salicylate- SA 100%, BA 16.3/27.3%
      • Potential dual function- handles multiple inputs
    • Reaction rates (P.h.1): Km(SA) 51.6uM, Km(BA) 1273uM, Kcat/Km(SA) 230 s-1M-1, Kcat/Km(BA) 3 s-1M-1:
    • Standard potential issues (transfer out of cell etc.)
  4. Characteristics of dual products-same smell etc.
  5. BSMT ported to E. coli
  6. Down-regulated "in planta" by ethylene (regulated spatially and temporally)
  7. References & Links

[http://openwetware.org/wiki/IGEM:MIT/2006/Notebook/2006-6-6 OWW Wiki]