Difference between revisions of "Part:BBa K779409"
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| − | + | The U6-TetO-Sc part encodes conditional expression of the short RNA sequence Sc. The mammalian RNA Pol III U6 promoter incorporates tetracycline-responsive elements that allow for tightly regulated RNA expression under specific conditions. In the presence of the Tet repressor (TetR), two Tet operator (TetO) sites become bound by the TetR and prevent RNA Pol III from binding, subsequently inhibiting transcription of the downstream sequence. In the absence of TetR, RNA transcription proceeds normally. The expression of the short Sc RNA sequence is to be used for in vivo RNA strand displacement of a downstream gate-output complex. | |
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Latest revision as of 20:58, 29 September 2012
U6-TetO-Sc MammoBlock
The U6-TetO-Sc part encodes conditional expression of the short RNA sequence Sc. The mammalian RNA Pol III U6 promoter incorporates tetracycline-responsive elements that allow for tightly regulated RNA expression under specific conditions. In the presence of the Tet repressor (TetR), two Tet operator (TetO) sites become bound by the TetR and prevent RNA Pol III from binding, subsequently inhibiting transcription of the downstream sequence. In the absence of TetR, RNA transcription proceeds normally. The expression of the short Sc RNA sequence is to be used for in vivo RNA strand displacement of a downstream gate-output complex.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]