Difference between revisions of "Part:BBa K779400"
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| − | + | The U6-TetO-FF1 part encodes conditional expression of the short hairpin RNA sequence FF1. The mammalian RNA Pol III U6 promoter incorporates tetracycline-responsive elements that allow for tightly regulated RNA expression under specific conditions. In the presence of the Tet repressor (TetR), two Tet operator (TetO) sites become bound by the TetR and prevent RNA Pol III from binding, subsequently inhibiting transcription of the downstream sequence. In the absence of TetR, RNA transcription proceeds normally. The short hairpin FF1 RNA sequence can be used as an RNA interference mechanism through its binding to a complementary region present in the transcript of another gene, thus inhibiting translation of that sequence and silencing expression of that gene. | |
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Revision as of 20:33, 29 September 2012
U6-TetO-FF1 MammoBlock
The U6-TetO-FF1 part encodes conditional expression of the short hairpin RNA sequence FF1. The mammalian RNA Pol III U6 promoter incorporates tetracycline-responsive elements that allow for tightly regulated RNA expression under specific conditions. In the presence of the Tet repressor (TetR), two Tet operator (TetO) sites become bound by the TetR and prevent RNA Pol III from binding, subsequently inhibiting transcription of the downstream sequence. In the absence of TetR, RNA transcription proceeds normally. The short hairpin FF1 RNA sequence can be used as an RNA interference mechanism through its binding to a complementary region present in the transcript of another gene, thus inhibiting translation of that sequence and silencing expression of that gene.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 248